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CD163 Protein (CD163) (AA 39-1121) (rho-1D4 tag)

Crystallography grade CD163 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3134950
  • Antigène Voir toutes CD163 Protéines
    CD163
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 39-1121
    Origine
    • 13
    • 6
    • 2
    • 1
    Souris
    Source
    • 9
    • 6
    • 3
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CD163 protéine est marqué à la rho-1D4 tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    VTNAPGEMKK ELRLAGGENN CSGRVELKIH DKWGTVCSNG WSMNEVSVVC QQLGCPTSIK ALGWANSSAG SGYIWMDKVS CTGNESALWD CKHDGWGKHN CTHEKDAGVT CSDGSNLEMR LVNSAGHRCL GRVEIKFQGK WGTVCDDNFS KDHASVICKQ LGCGSAISFS GSAKLGAGSG PIWLDDLACN GNESALWDCK HRGWGKHNCD HAEDVGVICL EGADLSLRLV DGVSRCSGRL EVRFQGEWGT VCDDNWDLRD ASVVCKQLGC PTAISAIGRV NASEGSGQIW LDNISCEGHE ATLWECKHQE WGKHYCHHRE DAGVTCSDGA DLELRLVGGG SRCAGIVEVE IQKLTGKMCS RGWTLADADV VCRQLGCGSA LQTQAKIYSK TGATNTWLFP GSCNGNETTF WQCKNWQWGG LSCDNFEEAK VTCSGHREPR LVGGEIPCSG RVEVKHGDVW GSVCDFDLSL EAASVVCREL QCGTVVSILG GAHFGEGSGQ IWGEEFQCSG DESHLSLCSV APPLDRTCTH SRDVSVVCSR YIDIRLAGGE SSCEGRVELK TLGAWGPLCS SHWDMEDAHV LCQQLKCGVA QSIPEGAHFG KGAGQVWSHM FHCTGTEEHI GDCLMTALGA PTCSEGQVAS VICSGNQSQT LLPCSSLSPV QTTSSTIPKE SEVPCIASGQ LRLVGGGGRC AGRVEVYHEG SWGTVCDDNW DMTDANVVCK QLDCGVAINA TGSAYFGEGA GAIWLDEVIC TGKESHIWQC HSHGWGRHNC RHKEDAGVIC SEFMSLRLTN EAHKENCTGR LEVFYNGTWG SIGSSNMSPT TVGVVCRQLG CADNGTVKPI PSDKTPSRPM WVDRVQCPKG VDTLWQCPSS PWKQRQASPS SQESWIICDN KIRLQEGHTD CSGRVEIWHK GSWGTVCDDS WDLNDAKVVC KQLGCGQAVK ALKEAAFGPG TGPIWLNEIK CRGNESSLWD CPAKPWSHSD CGHKEDASIQ CLPKMTSESH HGTGHPTLTA LLVCGAILLV LLIVFLLWTL KRRQIQRLTV SSRGEVLIHQ VQYQEMDSKA DDLDLLKSSG VIQRHTEKEN DNL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Cd163 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CD163 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CD163
    Autre désignation
    Cd163 (CD163 Produits)
    Synonymes
    DMBT1 Protein, CD163 Protein, CD163v2 Protein, CD163v3 Protein, M130 Protein, MM130 Protein, deleted in malignant brain tumors 1 protein-like Protein, CD163 molecule Protein, CD163 antigen Protein, LOC426820 Protein, CD163 Protein, Cd163 Protein
    Sujet
    Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH -dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity). {ECO:0000250}., After shedding, the soluble form (sCD163) may play an anti-inflammatory role. {ECO:0000250}.
    Poids moléculaire
    118.3 kDa Including tag.
    UniProt
    Q2VLH6
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