Patched 1 Protein (PTCH1) (AA 1-1434) (Strep Tag)

N° du produit ABIN3135358

Détail du produit

Antigène: Patched 1 (PTCH1)

Type de proteíne: Recombinant

Attributs du protein: AA 1-1434

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette Patched 1 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MASAGNAAGA LGRQAGGGRR RRTGGPHRAA PDRDYLHRPS YCDAAFALEQ ISKGKATGRK APLWLRAKFQ RLLFKLGCYI QKNCGKFLVV GLLIFGAFAV GLKAANLETN VEELWVEVGG RVSRELNYTR QKIGEEAMFN PQLMIQTPKE EGANVLTTEA LLQHLDSALQ ASRVHVYMYN RQWKLEHLCY KSGELITETG YMDQIIEYLY PCLIITPLDC FWEGAKLQSG TAYLLGKPPL RWTNFDPLEF LEELKKINYQ VDSWEEMLNK AEVGHGYMDR PCLNPADPDC PATAPNKNST KPLDVALVLN GGCQGLSRKY MHWQEELIVG GTVKNATGKL VSAHALQTMF QLMTPKQMYE HFRGYDYVSH INWNEDRAAA ILEAWQRTYV EVVHQSVAPN STQKVLPFTT TTLDDILKSF SDVSVIRVAS GYLLMLAYAC LTMLRWDCSK SQGAVGLAGV LLVALSVAAG LGLCSLIGIS FNAATTQVLP FLALGVGVDD VFLLAHAFSE TGQNKRIPFE DRTGECLKRT GASVALTSIS NVTAFFMAAL IPIPALRAFS LQAAVVVVFN FAMVLLIFPA ILSMDLYRRE DRRLDIFCCF TSPCVSRVIQ VEPQAYTEPH SNTRYSPPPP YTSHSFAHET HITMQSTVQL RTEYDPHTHV YYTTAEPRSE ISVQPVTVTQ DNLSCQSPES TSSTRDLLSQ FSDSSLHCLE PPCTKWTLSS FAEKHYAPFL LKPKAKVVVI LLFLGLLGVS LYGTTRVRDG LDLTDIVPRE TREYDFIAAQ FKYFSFYNMY IVTQKADYPN IQHLLYDLHK SFSNVKYVML EENKQLPQMW LHYFRDWLQG LQDAFDSDWE TGRIMPNNYK NGSDDGVLAY KLLVQTGSRD KPIDISQLTK QRLVDADGII NPSAFYIYLT AWVSNDPVAY AASQANIRPH RPEWVHDKAD YMPETRLRIP AAEPIEYAQF PFYLNGLRDT SDFVEAIEKV RVICNNYTSL GLSSYPNGYP FLFWEQYISL RHWLLLSISV VLACTFLVCA VFLLNPWTAG IIVMVLALMT VELFGMMGLI GIKLSAVPVV ILIASVGIGV EFTVHVALAF LTAIGDKNHR AMLALEHMFA PVLDGAVSTL LGVLMLAGSE FDFIVRYFFA VLAILTVLGV LNGLVLLPVL LSFFGPCPEV SPANGLNRLP TPSPEPPPSV VRFAVPPGHT NNGSDSSDSE YSSQTTVSGI SEELRQYEAQ QGAGGPAHQV IVEATENPVF ARSTVVHPDS RHQPPLTPRQ QPHLDSGSLS PGRQGQQPRR DPPREGLRPP PYRPRRDAFE ISTEGHSGPS NRDRSGPRGA RSHNPRNPTS TAMGSSVPSY CQPITTVTAS ASVTVAVHPP PGPGRNPRGG PCPGYESYPE TDHGVFEDPH VPFHVRCERR DSKVEVIELQ DVECEERPWG SSSN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Patched 1 (PTCH1)

Autre désignation: Ptch1 (PTCH1 Produits)

Synonymes: BCNS Protein, HPE7 Protein, NBCCS Protein, PTC Protein, PTC1 Protein, PTCH Protein, PTCH11 Protein, lep Protein, ptc1 Protein, ptc2 Protein, Ptch Protein, Ptch2 Protein, Ptc-1 Protein, XPtc1 Protein, patched Protein, patched1 Protein, A230106A15Rik Protein, Ptc Protein, Ptc1 Protein, mes Protein, patched 1 Protein, patched 1 L homeolog Protein, Protein patched homolog 1 Protein, PTCH1 Protein, ptch1 Protein, Ptch1 Protein, ptch1.L Protein, ptc-1 Protein

Sujet: Protein patched homolog 1 (PTC) (PTC1),FUNCTION: Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. {ECO:0000269|PubMed:21537345}.

Poids moléculaire: 159.3 kDa

UniProt: Q61115

Pathways: Signalisation Hedgehog, Carbohydrate Homeostasis, Tube Formation