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SEMA5A Protein (AA 22-1077) (rho-1D4 tag)

Crystallography grade SEMA5A Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3135527
  • Antigène Voir toutes SEMA5A Protéines
    SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 22-1077
    Origine
    • 8
    • 2
    Souris
    Source
    • 4
    • 3
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette SEMA5A protéine est marqué à la rho-1D4 tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    PETQDPAKCQ RAEHPVVSYK EIGPWLREFR AENAVDFSRL TFDPGQKELV VGARNYLFRL ELEDLSLIQA VEWECDEATK KACYSKGKSK EECQNYIRVL LVGGDRLFTC GTNAFTPVCT IRSLSNLTEI HDQISGMARC PYSPQHNSTA LLTASGELYA ATAMDFPGRD PAIYRSLGTL PPLRTAQYNS KWLNEPNFVS SYDIGNFTYF FFRENAVEHD CGKTVFSRPA RVCKNDIGGR FLLEDTWTTF MKARLNCSRP GEVPFYYNEL QGTFFLPELD LIYGIFTTNV NSIASSAVCV FNLSAISQAF NGPFKYQENS RSAWLPYPNP NPNFQCGTMD QGLYVNLTER NLQDAQKFIL MHEVVQPVTT VPSFMEDNSR FSHLAVDVVQ GRETLVHIIY LGTDYGTIKK VRAPLSQSSG SCLLEEIELF PERRSEPIRS LQILHSQSVL FVGLQEHVAK IPLKRCHFHQ TRSACIGAQD PYCGWDAVMK KCTSLEESLS MTQWDQSIPT CPTRNLTVDG SFGPWSPWTP CTHTDGTAVG SCLCRSRSCD RPAPQCGGWQ CEGPRMEITN CSRNGGWTPW TSWSPCSTTC GIGFQVRQRS CSNPTPRHGG RVCVGQNREE RYCNEHLLCP PHVFWTGWGP WERCTAQCGG GIQARRRTCE NGPDCAGSNV EYHPCNTNAC PELKKTTPWT PWTPVNISDN GGHYEQRFRY TCKARLPDPN LLEVGRQRIE MRYCSSDGTS GCSTDGLSGD FLRAGRYSAH TVNGAWSAWT SWSQCSRDCS RGIRNRKRVC NNPEPKFGGM PCLGPSLEFQ ECNILPCPVD GVWSCWSSWS KCSATCGGGH YMRTRSCSNP APAYGGDICL GLHTEEALCN TQTCPESWSE WSDWSVCDAS GTQVRARQCI LLFPVGSQCS GNTTESRPCV FDSNFIPEVS VARSSSVEEK RCGEFNMFHM FHMMAVGLSS SILGCLLTLL VYTYCQRYQQ QSHDATVIHP VSPAALNSSI TNHINKLDKY DSVEAIKAFN KNNLILEERN KYFNPHLTGK TYSNAYFTDL NNYDEY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Sema5a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product SEMA5A Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))
    Autre désignation
    Sema5a (SEMA5A Produits)
    Synonymes
    wu:fa95e03 Protein, wu:fi41a04 Protein, SEMAF Protein, semF Protein, 5930434A13 Protein, 9130201M22Rik Protein, AI464145 Protein, Semaf Protein, semaphorin 5A Protein, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A Protein, semaphorin-5A Protein, SEMA5A Protein, sema5a Protein, Sema5a Protein, LOC100516117 Protein
    Sujet
    Bifunctional axonal guidance cue regulated by sulfated proteoglycans, attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1 (By similarity). May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis. {ECO:0000250, ECO:0000269|PubMed:19850054}.
    Poids moléculaire
    119.6 kDa Including tag.
    UniProt
    Q62217
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