BOC Protein (AA 26-1110) (rho-1D4 tag)

N° du produit ABIN3135678

Détail du produit

Antigène: BOC (Brother Of CDO (BOC))

Type de proteíne: Recombinant

Attributs du protein: AA 26-1110

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette BOC protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: DLNEVPQVTV QPMSTVQKLG GTVILGCVVE PPWMNVTWRF NGKELNGSDD ALGVFITRGT LVIAALNNHT VGRYQCVARM PAGAVASVPA TVTLANLQDF KLDVQHVIEV DEGNTAVIAC HLPESHPKAQ VRYSVKQEWL EASRDNYLIM PSGNLQIVNA SQEDEGMYKC AAYNPVTQEV KTSGSGDRLR VRRSTAEAAR IIYPLEAQTV IVTKGQSLIL ECVASGIPPP RVTWAKDGSS IAAYNKTRFL LSNLLIDTTS EEDSGTYRCM ASNGVGDPGA AVILYNVQVF EPPEVTVELS QLVIPWGQSA KLTCEVRGNP PPSVLWLRNA VPLTSSQRLR LSRRALRVVS VGPEDEGVYQ CMAENAVGSA HAVVQLRTAR PDTTLRPGRD TKPIAATPPM PPSRPSRPDQ MLREQPGLVK PPTSSVQPTS LKCPGEEQVA PAEAPIILSS PRTSKTDSYE LVWRPRHEGS SRTPILYYVV KHRKVTNSSD DWTISGIPAN QHRLTLTRLD PGSLYEVEMA AYNCAGEGQT AMVTFRTGRR PKPEIVASKE QQIQRDDPGA SLQSSSQPDH GRLSPPEAPD RPTISTASET SVYVTWIPRG NGGFPIQSFR VEYKKLKKVG DWILATSAIP PSRLSVEITG LEKGISYKFR VRALNMLGES EPSAPSRPYV VSGYSGRVYE RPVAGPYITF TDAVNETTIM LKWMYIPASN NNTPIHGFYI YYRPTDSDND SDYKKDMVEG DRYWHSISHL QPETSYDIKM QCFNEGGESE FSNVMICETK ARKFSGQPGR PPPLTLAPPQ PPPLETMERP VGTGAMVARA SDLPYLIVGV VLGSIVLIIV TFIPFCLWRA WSKQKHTTDL GFPRSALLSS SCQYTMVPLE GLPGHQANGQ PYLGGVSGRA CVSRVHGSRG CPAATVGCPG RKPQQHCPGE LAQQREDTNS QLRQPIVSNG YDLQNQQVAR GPQCASGVGA FLYTLPDDST HQLLQPQDCC HLQKQPVTTC QTAVRRTSES PGLESSWDPP YHSGPRCCLG LVPVEEVDSS DSCQVGGGDW SSQHPSGTYT GQERGMRFSP SPSVHVSFET PPPTI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Boc Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: BOC (Brother Of CDO (BOC))

Autre désignation: Boc (BOC Produits)

Synonymes: TTN Protein, BOC Protein, MGC146299 Protein, LOC100226489 Protein, 4732455C11 Protein, mFLJ00376 Protein, CDON2 Protein, BOC cell adhesion associated, oncogene regulated L homeolog Protein, BOC cell adhesion associated, oncogene regulated Protein, biregional cell adhesion molecule-related/down-regulated by oncogenes (Cdon) binding protein Protein, boc.L Protein, BOC Protein, boc Protein, Boc Protein

Sujet: Component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. Promotes differentiation of myogenic cells.

Poids moléculaire: 119.7 kDa Including tag.

UniProt: Q6AZB0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development