CELF3 Protein (AA 1-465) (Strep Tag)

N° du produit ABIN3153964

Détail du produit

Antigène: CELF3 (CUGBP, Elav-Like Family Member 3 (CELF3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-465

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette CELF3 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MKEPDAIKLF VGQIPRHLEE KDLKPIFEQF GRIFELTVIK DKYTGLHKGC AFLTYCARDS ALKAQSALHE QKTLPGMNRP IQVKPADSES RGDRKLFVGM LGKQQTDEDV RKMFEPFGTI DECTVLRGPD GTSKGCAFVK FQTHAEAQAA INTLHSSRTL PGASSSLVVK FADTEKERGL RRMQQVATQL GMFSPIALQF GAYSAYTQAL MQQQAALVAA HSAYLSPMAT MAAVQMQHMA AISANGLIAT PITPSSGTST PPAIAATPVS AIPAALGVNG YSPVPTQPTG QPAPDALYPN GVHPYPAQSP AAPVDPLQQA YAGMQHYTAA YPAAYSLVAP AFPQPPALVA QQPPPPPQQQ QQQQQQQQQQ QQQREGPDGC NIFIYHLPQE FTDSEILQMF VPFGHVISAK VFVDRATNQS KCFGFVSFDN PASAQAAIQA MNGFQIGMKR LKVQLKRPKD ANRPY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CELF3 (CUGBP, Elav-Like Family Member 3 (CELF3))

Autre désignation: Celf3 (CELF3 Produits)

Synonymes: BRUNOL1 Protein, CAGH4 Protein, ERDA4 Protein, TNRC4 Protein, 4930415M08Rik Protein, Tnrc4 Protein, etr1 Protein, cagh4 Protein, erda4 Protein, etr-1 Protein, tnrc4 Protein, brunol1 Protein, CELF-3A Protein, tnrc4-a Protein, RGD1563168 Protein, celf3 Protein, tnrc4-b Protein, CUGBP Elav-like family member 3 Protein, CUGBP, Elav-like family member 3 Protein, CUGBP, Elav-like family member 3 L homeolog Protein, cugbp, Elav-like family member 3a Protein, CUGBP, Elav-like family member 3 S homeolog Protein, CELF3 Protein, Celf3 Protein, celf3 Protein, celf3.L Protein, celf3a Protein, celf3.S Protein

Sujet: CUGBP Elav-like family member 3 (CELF-3) (Bruno-like protein 1) (CUG-BP- and ETR-3-like factor 3) (ELAV-type RNA-binding protein 1) (ETR-1) (RNA-binding protein BRUNOL-1) (Trinucleotide repeat-containing gene 4 protein),FUNCTION: RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity). {ECO:0000250}.

Poids moléculaire: 50.5 kDa

UniProt: Q8CIN6