ABCA6 Protein (AA 1-1624) (Strep Tag)

N° du produit ABIN3136597

Détail du produit

Antigène: ABCA6 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 6 (ABCA6))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1624

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ABCA6 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MKELSVHVRQ QTRALLHKIL LKKWRRKRES LLEWSIPIII GLHMGLFSYL ARNIQVLEVP PQDLGSLNEF NGSSLVVVYT PISNITQQIM NKTTFAPTMK GTRIIGVPSI EDLDEVLLHN IPDALGVIFN DSFSYQLKVL RMYGNPFLKE DLLAHCWDTH SQAFCSLSKY WERGFVALQT AINAGIIEVT TNHSVMEELM SIDGINMKTL PFIPRDLSDY EIFILFCLLY FSSFIYFASS NVTKERKQCK EVMKVMGLQD SAFWLSWGLI YVGFIFIISI FIAIIITSTQ IIMMTGFLVI FTLFFLYGLS LIAVTFLMAV LLQKAVLTNL IVLFFTLFWG CVGFTVLHKE LPPSLEWVLS IFSPFAFTSG MAKVIFQDYN LNGVVFPDPS GESYVMIAVF FILAFDSLLY LVLALYFDKI LLYGAEHRSA PLFFLNPTSC FRKTANRNKV IERDLDPELP SDEYFEPVDP EYQGKEAIRI RNIKKEYKGK SGKVKALKGL FLDIYESQIT AILGHSGAGK SSLLNILSGL YVPTAGSVTV YNKNLSDMQD LKEIRKAIGV CPQHNVQFDA LTVKENLTLF AKIKGILPQD VEQEVQQILS ELDMQNIRDD LAEHLSEGQK RKLTFGIATV GDPQILLLDE PTVGLDPFSR QRIWGFLKER RADHVILFST QFMDEADILA DRKVLIANGA LKCTGSSVFL KRKWGLGYHL SLFMDETCDS ERLTSFINHH IPYAKLKAKT KEKLVYILPL ERTSEFPEFF SDLDKYSGQG LMSYEVSMST LNDVFLNLEG EPSTKQDFEK RETATDSESL NDMEVAYPSL SQVQETVSTM SLWRMQVCAI ARLRILKLKR ERKAFLIILL LLGIALLPLV IEYVANALLE VKNNWEFKTD LYFLSPGQLP QGLRTSLLVI NNTESNIEDF LQSLKHQNIV LEVDDFENRN ATNSLSYNGA IIVSGRQKDY RFSAVCNTKR LHCFPILMNV ISNGILHMLN HTQYIRIKED IFSPFIVLVW TGIQETCLFI LCVICSLSPH IAMSSVSDYK KKADSQLWIS GLYPSAYWCG QAVVDISLFS GMLLTSYFTS YTSKLLNIDM TSEIVFSVIV LALGCAASLV FLTYVISFVF GKRKKNSTLW SICFLLVIAI TFEKVANGPF NEALVISATM LVPSFALNGL LVVLEMRAYQ YYIEFEEIKH GLSAVDLLLC LIPYIHTLLF IFVLRCLELK YGKNVVRRDP IFRIAPQSLK AQPNPEEPID EDENVQAERL RTSDALSTPN LDEKPVIIAS CLHKEYAGQK KHCCSRRTRN MAVRNVSFCV NKGEILGLLG PDGAGKSSSI RMIAGITKPT AGQVELKRLS SAVGHQGDSR AEFGYCPQEN GLWPNLTVKE HLELYAAVKG LRKEDAVVAI SRLVNALKLH DQLNVQVQNL VAGATRKLCF VLSILGNSPV LILDEPSTGL DVSGKHQVWQ AIQAVVKDNE KGVLLSTHDL AEAEALCDRA AIMVSGRLRC IGPIQHLKRK FGQDYVLELR VKDVSQEPLV HREILKLFPQ AARQDRCFSL LTYKLPVTDV HPLSQAFHKL EAVKHGFDLE DYSLSQCTLD RVILELSKEQ ELGTVYEEAD MTLGRKLLPP SDEL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ABCA6 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 6 (ABCA6))

Autre désignation: Abca6 (ABCA6 Produits)

Synonymes: ABCA6 Protein, EST155051 Protein, 6330565N06Rik Protein, ATP binding cassette subfamily A member 6 Protein, ATP-binding cassette sub-family A member 9 Protein, ATP-binding cassette, sub-family A (ABC1), member 6 Protein, Abca6 Protein, ABCA6 Protein, LOC100545599 Protein

Sujet: ATP-binding cassette sub-family A member 6 (EC 7.6.2.-),FUNCTION: Probable transporter which may play a role in macrophage lipid transport and homeostasis. {ECO:0000250|UniProtKB:Q8N139}.

Poids moléculaire: 183.3 kDa

UniProt: Q8K441