TPCN1 Protein (AA 1-817) (Strep Tag)

N° du produit ABIN3129789

Détail du produit

Antigène: TPCN1 (Two Pore Segment Channel 1 (TPCN1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-817

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TPCN1 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Séquence: MAVSLDDDVP LILTLDEAES APLPPSNSLG QEQLPSKNGG SHSIHNSQVP SLVSGADSPP SSPTGHNWEM NYQEAAIYLQ EGQNNDKFFT HPKDARALAA YLFVHNHFFY MMELLTALLL LLLSLCESPA VPVLKLHTYV HATLELFALM VVVFELCMKL RWLGFHTFVR HKRTMVKTSV LVVQFIEAIV VLVRQTSHVR VTRALRCIFL VDCRYCGGVR RNLRQIFQSL PPFMDILLLL LFFMIIFAIL GFYLFSTNPS DPYFSTLENS IVNLFVLLTT ANFPDVMMPS YSRNPWSCVF FIVYLSIELY FIMNLLLAVV FDTFNDIEKH KFKSLLLHKR TAIQHAYGLL ASQRRPAGIS YRQFEGLMRF YKPRMSARER FLTFKALNQS NTPLLSLKDF YDIYEVAALQ WKAKRNRQHW FDELPRTAFL IFKGINILVN SKAFQYFMYL VVAVNGVWIL VETFMLKGGN FTSKHVPWSY LVFLTIYGVE LFMKVAGLGP VEYLSSGWNL FDFSVTAFAF LGLLALTLNM EPFYFIVVLR PLQLLRLFKL KKRYRNVLDT MFELLPRMAS LGLTLLTFYY SFAIVGMEFF NGRLTPNCCN TSTVADAYRF INHTVGNKTK VEEGYYYLNN FDNILNSFVT LFELTVVNNW YIIMEGVTSQ TSHWSRLYFM TFYIVTMVVM TIIVAFILEA FVFRMNYSRK SQDSEVDSGI VIEKEMSKEE LMAVLELYRE ERGTSSDVTR LLDTLSQMEK YQQNSMVFLG RRSRTKSDLS LKMYQEEIQE WYEEHAREQE QQKLRGSVPG PAAQQPPGSR QRSQTVT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TPCN1 (Two Pore Segment Channel 1 (TPCN1))

Autre désignation: Tpcn1 (TPCN1 Produits)

Synonymes: ATCCH1 Protein, ATTPC1 Protein, CALCIUM CHANNEL 1 Protein, FATTY ACID OXYGENATION UPREGULATED 2 Protein, FOU2 Protein, T5L23.5 Protein, two-pore channel 1 Protein, 5730403B01Rik Protein, Tpc1 Protein, mKIAA1169 Protein, TPC1 Protein, si:dkey-125i10.5 Protein, two pore segment channel 1 Protein, two-pore channel 1 Protein, two pore channel 1 Protein, Tpcn1 Protein, TPC1 Protein, TPCN1 Protein, tpcn1 Protein

Sujet: Two pore calcium channel protein 1 (Voltage-dependent calcium channel protein TPC1),FUNCTION: Intracellular channel initially characterized as a non-selective Ca(2+)-permeable channel activated by NAADP (nicotinic acid adenine dinucleotide phosphate), it is also a voltage-gated highly-selective Na(+) channel activated directly by PI(3,5)P2 (phosphatidylinositol 3,5-bisphosphate) that senses pH changes and confers electrical excitability to organelles (PubMed:24776928, PubMed:23394946, PubMed:23063126). Localizes to the early and recycling endosomes membranes where it plays a role in the uptake and processing of proteins and regulates organellar membrane excitability, membrane trafficking and pH homeostasis (PubMed:28855648). Ion selectivity is not fixed but rather agonist-dependent and under defined ionic conditions, can be readily activated by both NAADP and PI(3,5)P2 (By similarity). Required for mTOR-dependent nutrient sensing (PubMed:23394946). {ECO:0000250|UniProtKB:Q8NHX9, ECO:0000269|PubMed:23063126, ECO:0000269|PubMed:23394946, ECO:0000269|PubMed:24776928, ECO:0000269|PubMed:28855648}.

Poids moléculaire: 94.5 kDa

UniProt: Q9EQJ0