MBTPS1 Protein (AA 187-1052) (rho-1D4 tag)
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- Antigène Voir toutes MBTPS1 Protéines
- MBTPS1 (Membrane-Bound Transcription Factor Peptidase, Site 1 (MBTPS1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 187-1052
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Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette MBTPS1 protéine est marqué à la rho-1D4 tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
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RAIPRQVAQT LQADVLWQMG YTGANVRVAV FDTGLSEKHP HFKNVKERTN WTNERTLDDG LGHGTFVAGV IASMRECQGF APDAELHIFR VFTNNQVSYT SWFLDAFNYA ILKKMDVLNL SIGGPDFMDH PFVDKVWELT ANNVIMVSAI GNDGPLYGTL NNPADQMDVI GVGGIDFEDN IARFSSRGMT TWELPGGYGR VKPDIVTYGA GVRGSGVKGG CRALSGTSVA SPVVAGAVTL LVSTVQKREL VNPASVKQAL IASARRLPGV NMFEQGHGKL DLLRAYQILS SYKPQASLSP SYIDLTECPY MWPYCSQPIY YGGMPTIVNV TILNGMGVTG RIVDKPEWRP YLPQNGDNIE VAFSYSSVLW PWSGYLAISI SVTKKAASWE GIAQGHIMIT VASPAETELH SGAEHTSTVK LPIKVKIIPT PPRSKRVLWD QYHNLRYPPG YFPRDNLRMK NDPLDWNGDH VHTNFRDMYQ HLRSMGYFVE VLGAPFTCFD ATQYGTLLLV DSEEEYFPEE IAKLRRDVDN GLSLVIFSDW YNTSVMRKVK FYDENTRQWW MPDTGGANIP ALNELLSVWN MGFSDGLYEG EFVLANHDMY YASGCSIAKF PEDGVVITQT FKDQGLEVLK QETAVVENVP ILGLYQIPSE GGGRIVLYGD SNCLDDSHRQ KDCFWLLDAL LQYTSYGVTP PSLSHSGNRQ RPPSGAGLAP PERMEGNHLH RYSKVLEAHL GDPKPRPLPA CPHLSWAKPQ PLNETAPSNL WKHQKLLSID LDKVVLPNFR SNRPQVRPLS PGESGAWDIP GGIMPGRYNQ EVGQTIPVFA FLGAMVALAF FVVQISKAKS RPKRRRPRAK RPQLAQQAHP ARTPSV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Mbtps1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin-free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product MBTPS1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- MBTPS1 (Membrane-Bound Transcription Factor Peptidase, Site 1 (MBTPS1))
- Autre désignation
- Mbtps1 (MBTPS1 Produits)
- Synonymes
- PCSK8 Protein, S1P Protein, SKI-1 Protein, 0610038M03Rik Protein, AV003995 Protein, Ski1 Protein, mKIAA0091 Protein, S1p Protein, Ski-1 Protein, membrane bound transcription factor peptidase, site 1 Protein, membrane-bound transcription factor peptidase, site 1 Protein, MBTPS1 Protein, Mbtps1 Protein
- Sujet
- Serine protease that catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs). Other known substrates are BDNF, GNPTAB and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg-Ser-Val-Leu (SERBP-2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes (By similarity). {ECO:0000250}.
- Poids moléculaire
- 97.4 kDa Including tag.
- UniProt
- Q9WTZ2
- Pathways
- ER-Nucleus Signaling
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