FCGRT & B2M (AA 1-297), (Extracellular Domain) (Active) protein (His tag)

Détails pour le produit réf. ABIN3214466
Nom de la protéine
Attributs du protein
AA 1-297, Extracellular Domain
3
2
2
1
1
1
Origine
Humain
2
1
1
1
Source
Human Cells
5
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
His tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Attributs du produit The recombinant heterodimer of human FCGRT/B2M comprises 385 (285 + 100) amino acids and has a calculated molecular mass of 43.5 (31.8 + 11.7) kDa. The apparent molecular mass of rh FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of human FCGRT (P55899) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1, A DNA sequence encoding the human B2M (P61769) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified.
Predicted N-Term: Ala 24 & Ile 21
Pureté > 95 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to bind human IgG1 in a functional ELISA.
Nom de la protéine
Sujet Synonyms: alpha-chain,FCRN
Poids moléculaire 12 kDa
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Date de péremption 12 months
Images (Fournisseur)
SDS-PAGE (SDS) image for FCGRT & B2M (AA 1-297), (Extracellular Domain) (Active) protein (His tag) (ABIN3214466) FCGRT & B2M (AA 1-297), (Extracellular Domain) (Active) protein (His tag)
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