Alanyl (Membrane) Aminopeptidase (ANPEP) (Met603Ile-Mutant), (Metalloprotease Domain) (Active) protein (His tag)

Détails pour le produit réf. ABIN3216379
Nom de la protéine
Attributs du protein
Metalloprotease Domain, Met603Ile-Mutant
1
1
1
1
1
1
1
1
1
1
1
1
1
Origine
Humain
10
4
1
1
Source
Human Cells
4
3
3
2
2
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
Cette CD13 protéine est marqué à la His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Attributs du produit The recombinant human ANPEP protein consists of 910 amino acids and has a predicted molecular mass of 104.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhANPEP is approximately 135-140 kDa due to glycosylation.

Protein Structure: A DNA sequence encoding the metalloprotease domain (Lys 69-Lys 967,603 Met/Ile) of the mature form of human ANPEP (NP_001141.2) was expressed with N-terminal signal peptide and C-terminal polyhistidine tag.
Predicted N-Term: Lys 69
Pureté > 97 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC) . The specific activity is >2,500 pmoles/min/μg.
Nom de la protéine
Sujet Synonyms: APN,CD13,GP150,LAP1,P150,PEPN
Poids moléculaire 104.2 kDa, 135-140 kDa
NCBI Accession NP_001141
Pathways Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.3
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Date de péremption 12 months
Produit citée dans: Li, Hao, He, Meng, Li, Xue, Zhang, Li, Zhang, Zhang: "Recombinant IFN-?2a-NGR exhibits higher inhibitory function on tumor neovessels formation compared with IFN-?2a in vivo and in vitro." dans: Cytotechnology, 2014 (PubMed).