Intercellular Adhesion Molecule 3 (ICAM3) (AA 1-485), (Extracellular Domain) (Active) protein (His tag)

Détails pour le produit réf. ABIN3216383
Nom de la protéine
  • CD50
  • CDW50
  • ICAM-R
  • ICAM-3
  • ICAM3
  • intercellular adhesion molecule 3
  • ICAM3
  • Icam3
Attributs du protein
AA 1-485, Extracellular Domain
2
2
1
1
1
1
1
Origine
Humain
10
1
1
Source
Human Cells
4
2
2
2
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
His tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Attributs du produit The recombinant human ICAM3 consists of 467 amino acids after removal of the signal peptide and predicts a molecular mass of 50.8 kDa. By SDS-PAGE, the apparent molecular mass of rh ICAM3 is approximately 100-120 kDa due to the glycosylation.

Protein Structure: A DNA sequence encoding the extracellular domain of human ICAM3 (NP_002153.2) (Met 1-His 485) was expressed, with the C-terminal fused polyhistidine tag.
Predicted N-Term: Gln 30
Pureté > 92 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When cells are added to ICAM3-coated plates (12.5 μg/mL , 100 μL/well), approximately >25% cells will adhere specifically.
Nom de la protéine
Sujet Synonyms: CD50,CDW50,ICAM-R
Poids moléculaire 50.8 kDa, 100-120 kDa
NCBI Accession NP_002153
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Date de péremption 12 months
Images (Fournisseur)
 image for Intercellular Adhesion Molecule 3 (ICAM3) (AA 1-485), (Extracellular Domain) (Active) protein (His tag) (ABIN3216383) Intercellular Adhesion Molecule 3 (ICAM3) (AA 1-485), (Extracellular Domain) (Active) protein (His tag)
Background publications Jolly, Mitar, Sattentau: "Adhesion molecule interactions facilitate human immunodeficiency virus type 1-induced virological synapse formation between T cells." dans: Journal of virology, Vol. 81, Issue 24, pp. 13916-21, 2007 (PubMed).

Pino-Otín, Juan, de la Fuente, Viñas, Martínez-Cáceres, Fernández, Miralles, Vilella, Yagüe, Vives: "CD50 (intercellular adhesion molecule-3) is expressed at higher levels on memory than on naive human T cells but induces a similar calcium mobilization on both subsets." dans: Tissue antigens, Vol. 46, Issue 1, pp. 32-44, 1995 (PubMed).

Simmons: "The role of ICAM expression in immunity and disease." dans: Cancer surveys, Vol. 24, pp. 141-55, 1995 (PubMed).

Fawcett, Holness, Needham, Turley, Gatter, Mason, Simmons: "Molecular cloning of ICAM-3, a third ligand for LFA-1, constitutively expressed on resting leukocytes." dans: Nature, Vol. 360, Issue 6403, pp. 481-4, 1992 (PubMed).

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