Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 1-502) (Active) protein (His tag)

Détails pour le produit réf. ABIN3216384
Nom de la protéine
  • ALP
  • iap
  • alpl
  • AP-TNAP
  • APTNAP
  • HOPS
  • TNAP
  • TNSALP
  • Akp-2
  • Akp2
  • PHOA
  • TNS-AP
  • alkaline phosphatase, liver/bone/kidney L homeolog
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, intestinal, gene 2
  • alkaline phosphatase, liver/bone/kidney pseudogene
  • alpl.L
  • ALPL
  • alpi.2
  • LOC100031702
  • Alpl
Attributs du protein
AA 1-502
2
2
2
1
1
1
1
1
1
Origine
Humain
9
7
1
Source
Human Cells
6
3
2
2
2
2
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
Cette ALPL protéine est marqué à la His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Attributs du produit The recombinant human ALPL consists of 496 amino acids and predicts a molecular mass of 55 kDa. As a result of glycosylation, rhALPL migrates as approximately 65 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human tissue-nonspecific alkaline phosphatase (NP_000469.3) (Met 1-Ser 502) was expressed with a C-terminal polyhistidine tag.
Predicted N-Term: Leu 18
Pureté > 95 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).
The specific activity is > 50, 000 pmoles/min/μg.
Nom de la protéine
Sujet Synonyms: AP-TNAP,APTNAP,HOPS,TNAP,TNSALP
Poids moléculaire 55 kDa, 65 kDa
NCBI Accession NP_000469
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 25 mM Tris, 0.15M NaCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Date de péremption 12 months
Images (Fournisseur)
 image for Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 1-502) (Active) protein (His tag) (ABIN3216384) Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 1-502) (Active) protein (His tag)
Background publications Peach, Zhang, Wordsworth: "Mutations of the tissue non-specific alkaline phosphatase gene (TNAP) causing a non-lethal case of perinatal hypophosphatasia." dans: Rheumatology (Oxford, England), Vol. 46, Issue 6, pp. 1037-40, 2007

Peach, Zhang, Wordsworth: "Mutations of the tissue non-specific alkaline phosphatase gene (TNAP) causing a non-lethal case of perinatal hypophosphatasia." dans: Rheumatology (Oxford, England), Vol. 46, Issue 6, pp. 1037-40, 2007 (PubMed).

Le Du, Millan: "Structural evidence of functional divergence in human alkaline phosphatases." dans: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49808-14, 2002 (PubMed).

Mornet: "Hypophosphatasia: the mutations in the tissue-nonspecific alkaline phosphatase gene." dans: Human mutation, Vol. 15, Issue 4, pp. 309-15, 2000 (PubMed).

Henthorn, Raducha, Fedde, Lafferty, Whyte: "Different missense mutations at the tissue-nonspecific alkaline phosphatase gene locus in autosomal recessively inherited forms of mild and severe hypophosphatasia." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 20, pp. 9924-8, 1992 (PubMed).

Harris: "The human alkaline phosphatases: what we know and what we don't know." dans: Clinica chimica acta; international journal of clinical chemistry, Vol. 186, Issue 2, pp. 133-50, 1990 (PubMed).

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