Histidine-Rich Glycoprotein (HRG) (AA 1-525) (Active) protein (His tag)

Détails pour le produit réf. ABIN3216389
Nom de la protéine
Attributs du protein
AA 1-525
4
2
1
1
1
Origine
Humain
8
4
3
1
Source
Human Cells
5
5
2
2
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
His tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Attributs du produit The secreted recombinant human HPRG comprises 518 amino acids with a predicted molecular mass of 59 kDa. As a result of glycosylation, rhHPRG migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human HPRG (NP_000403.1) (Met 1-Lys 525) with a C-terminal polyhistidine tag was expressed.
Predicted N-Term: Val 19
Pureté > 97 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. (Lamb-Wharton, R.J. and W.T. Morgen, 1993, Cell Immunol. 152: 544) . Human HPRG immobilized at 1 μg/ml (100 μl/well) will induce >65% MOLT4 cell adhesion (1 x 10E5 cells/well) in the presence of 7.5 μg/ml Concanvalin A.
Nom de la protéine
Sujet Synonyms: HPRG,HRGP,THPH11
Poids moléculaire 59 kDa, 75-80 kDa
NCBI Accession NP_000403
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Date de péremption 12 months