Kallikrein 1 (KLK1) (AA 1-261) (Active) protein (His tag)

Détails pour le produit réf. ABIN3216408
Nom de la protéine
  • KLKR
  • Klk6
  • hK1
  • klkr
  • kallikrein
  • 0610007D04Rik
  • Kal
  • Klk1b6
  • mGk-6
  • kallikrein 1
  • kallikrein 1 L homeolog
  • KLK1
  • klk1.L
  • LOC100101620
  • Klk1
Attributs du protein
AA 1-261
3
3
2
2
1
1
1
1
1
Origine
Souris
19
4
3
1
Source
Human Cells
10
5
4
2
2
2
1
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
Cette Kallikrein 1 protéine est marqué à la His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Attributs du produit The recombinant mouse KLK1 comprises 254 amino acids and has a predicted molecular mass of 28.3 kDa. The apparent molecular mass of the protein is approximately 36 kDa in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the mouse KLK1 (P15947) (Met1-Asp261) was expressed with a C-terminal polyhistidine tag.
Predicted N-Term: Pro 19
Pureté > 90 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin(PFR-AMC).
The specific activity is >6, 000 pmol/min/μg.
(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Nom de la protéine
Sujet Synonyms: 0610007D04Rik,Kal,Klk1b6,Klk6,mGk-6
Poids moléculaire 28.3 kDa, 36 kDa
UniProt P15947
Pathways Système du Complément
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 25 mM Tris, 5 mM CaCl2, 0.15 M NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Date de péremption 12 months
Images (Fournisseur)
 image for Kallikrein 1 (KLK1) (AA 1-261) (Active) protein (His tag) (ABIN3216408) Kallikrein 1 (KLK1) (AA 1-261) (Active) protein (His tag)
Background publications Clements, Hooper, Dong, Harvey: "The expanded human kallikrein (KLK) gene family: genomic organisation, tissue-specific expression and potential functions." dans: Biological chemistry, Vol. 382, Issue 1, pp. 5-14, 2001 (PubMed).

Bourgeois, Brillard-Bourdet, Deperthes, Juliano, Juliano, Tremblay, Dubé, Gauthier: "Serpin-derived peptide substrates for investigating the substrate specificity of human tissue kallikreins hK1 and hK2." dans: The Journal of biological chemistry, Vol. 272, Issue 47, pp. 29590-5, 1997 (PubMed).

Henttu, Lukkarinen, Vihko: "Expression of the gene coding for human prostate-specific antigen and related hGK-1 in benign and malignant tumors of the human prostate." dans: International journal of cancer. Journal international du cancer, Vol. 45, Issue 4, pp. 654-60, 1990 (PubMed).