5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 1-549) (Active) protein (His tag)

Détails pour le produit réf. ABIN3216411
Nom de la protéine
  • CALJA
  • CD73
  • E5NT
  • NT
  • NT5
  • NTE
  • eN
  • eNT
  • zgc:63784
  • NT5E
  • MGC114869
  • PSPTO2277
  • 2210401F01Rik
  • AI447961
  • Nt5
  • 5'-nucleotidase ecto
  • 5'-nucleotidase, ecto (CD73)
  • 5'-nucleotidase
  • 5' nucleotidase, ecto
  • NT5E
  • nt5e
  • nt5e.S
  • PSPTO_2277
  • CBU_1233
  • MCA1322
  • Nt5e
Attributs du protein
AA 1-549
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Origine
Rat (Rattus)
18
7
4
1
1
Source
Human Cells
13
9
5
2
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
Cette CD73 protéine est marqué à la His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Attributs du produit The recombinant rat NT5E comprises 532 amino acids and predicts a molecular mass of 59.4 kDa. The apparent molecular mass of the recombinant protein is approximately 59 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Structure: A DNA sequence encoding the rat NT5E(Q66HL0) (Met1-Lys549) was expressed with a polyhistidine tag at the C-terminus.
Predicted N-Term: Trp 29
Pureté > 90 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit .
The specific activity is >15,000 pmol/min/μg.
Nom de la protéine
Poids moléculaire 59.4 kDa, 59 kDa
UniProt Q66HL0
Pathways Synaptic Membrane, Ribonucleoside Biosynthetic Process
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Date de péremption 12 months
Images (Fournisseur)
 image for 5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 1-549) (Active) protein (His tag) (ABIN3216411) 5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 1-549) (Active) protein (His tag)
Background publications Grünewald, Ridley: "CD73 represses pro-inflammatory responses in human endothelial cells." dans: Journal of inflammation (London, England), Vol. 7, Issue 1, pp. 10, 2010 (PubMed).

Louis, Robinson, MacManus, Karhausen, Scully, Colgan: "Control of IFN-alphaA by CD73: implications for mucosal inflammation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 6, pp. 4246-55, 2008 (PubMed).

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