Serine (Or Cysteine) Peptidase Inhibitor, Clade C (Antithrombin), Member 1 (SERPINC1) (AA 1-464) (Active) protein (His tag)

Détails pour le produit réf. ABIN3216412
Nom de la protéine
  • AT3
  • AT3D
  • ATIII
  • THPH7
  • SERPINC1
  • DKFZp470C1733
  • AI114908
  • At-3
  • At3
  • ANTITHROMBIN, AT-III
  • AT-III
  • serpin family C member 1
  • antithrombin-III
  • serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
  • SERPINC1
  • CpipJ_CPIJ000472
  • CpipJ_CPIJ013111
  • Serpinc1
Attributs du protein
AA 1-464
5
3
2
1
1
1
1
Origine
Cynomolgus
22
5
4
2
1
1
1
Source
Human Cells
14
8
2
2
2
1
1
1
1
1
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
His tag
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Attributs du produit The recombinant cynomolgus SerpinC1 consists of 441 amino acids and has a calculated molecular mass of 50.2 kDa. The apparent molecular mass of the recombinant protein is approximately 55-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Structure: A DNA sequence encoding the Cynomolgus (Macaca fascicularis) SerpinC1 (Met 1-Ser 464) was fused with a polyhistidine tag at the C-terminus.
Predicted N-Term: Ser 35
Pureté > 95 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC.
The IC50 value is <5 nM.
Nom de la protéine
Sujet Synonyms: SerpinC1
Poids moléculaire 50.2 kDa, 55-60 kDa
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Date de péremption 12 months
Images (Fournisseur)
 image for Serine (Or Cysteine) Peptidase Inhibitor, Clade C (Antithrombin), Member 1 (SERPINC1) (AA 1-464) (Active) protein (His tag) (ABIN3216412) Serine (Or Cysteine) Peptidase Inhibitor, Clade C (Antithrombin), Member 1 (SERPINC1) (AA 1-464) (Active) protein (His tag)
Background publications Saito, Minamiya, Kalina, Saito, Ogawa: "Effect of antithrombin III on neutrophil deformability." dans: Journal of leukocyte biology, Vol. 78, Issue 3, pp. 777-84, 2005 (PubMed).

Oelschläger, Römisch, Staubitz, Stauss, Leithäuser, Tillmanns, Hölschermann: "Antithrombin III inhibits nuclear factor kappaB activation in human monocytes and vascular endothelial cells." dans: Blood, Vol. 99, Issue 11, pp. 4015-20, 2002 (PubMed).

Chuang, Swanson, Raja, Bock, Olson et al.: "The antithrombin P1 residue is important for target proteinase specificity but not for heparin activation of the serpin. Characterization of P1 antithrombin variants with altered proteinase ..." dans: Biochemistry, Vol. 40, Issue 22, pp. 6670-9, 2001 (PubMed).

Perry: "Antithrombin and its inherited deficiencies." dans: Blood reviews, Vol. 8, Issue 1, pp. 37-55, 1994 (PubMed).

Chandra, Stackhouse, Kidd, Woo: "Isolation and sequence characterization of a cDNA clone of human antithrombin III." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 7, pp. 1845-8, 1983 (PubMed).

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