Vaccinia Related Kinase 1 (VRK1) (AA 1-396) Protéine

Détails pour le produit réf. ABIN3216426
Nom de la protéine
  • vrk1
  • MGC64390
  • MGC75762
  • VRK1
  • PCH1
  • PCH1A
  • 51PK
  • wu:fe16f06
  • zgc:56266
  • vaccinia related kinase 1 L homeolog
  • vaccinia related kinase 1
  • Serine/threonine-protein kinase VRK1
  • vrk1.L
  • vrk1
  • VRK1
  • vrk-1
  • Vrk1
Attributs du protein
AA 1-396
7
1
1
1
1
1
Origine
Humain
12
1
1
Source
Baculovirus infected Insect Cells
5
3
2
2
1
1
Type de proteíne
Recombinant
Application
SDS-PAGE (SDS)
Options
Attributs du produit The secreted recombinant human VRK1 consists of 398 amino acids and predicts a molecular mass of 45.6 KDa. The apparent molecular mass of the protein is approximately 47 KDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Structure: A DNA sequence encoding the human VRK1 ( Q99986 ) (Met 1-Lys 396) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Predicted N-Term: Gly
Pureté > 95 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Kinase activity untested
Nom de la protéine
Sujet Synonyms: PCH1,PCH1A
UniProt Q99986
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Date de péremption 12 months
Images (Fournisseur)
 image for Vaccinia Related Kinase 1 (VRK1) (AA 1-396) protein (ABIN3216426) Vaccinia Related Kinase 1 (VRK1) (AA 1-396) protein
Background publications Lopez-Borges, Lazo: "The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein." dans: Oncogene, Vol. 19, Issue 32, pp. 3656-64, 2000

Lopez-Borges, Lazo: "The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein." dans: Oncogene, Vol. 19, Issue 32, pp. 3656-64, 2000 (PubMed).

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