Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-469) Protéine

Détails pour le produit réf. ABIN3216431
Nom de la protéine
  • CaMK IV
  • CaMK-GR
  • IV
  • caMK
  • CAMK4
  • A430110E23Rik
  • AI666733
  • CaMKIV
  • CaMKIV/Gr
  • D18Bwg0362e
  • Ccdpk
  • RATCCDPK
  • camk4-A
  • wu:fj42a03
  • zgc:110275
  • calcium/calmodulin dependent protein kinase IV
  • calcium/calmodulin-dependent protein kinase IV
  • calcium/calmodulin-dependent protein kinase IV L homeolog
  • CAMK4
  • Camk4
  • camk4.L
  • camk4
Attributs du protein
AA 1-469
4
2
1
1
Origine
Souris
11
2
1
Source
Baculovirus infected Insect Cells
4
3
2
2
1
1
1
Type de proteíne
Recombinant
Application
SDS-PAGE (SDS)
Options
Attributs du produit The recombinant mouse CAMK4 consists of 471 amino acids and predicts a molecular mass of 52.7 KDa. It migrates as an approximately 55 KDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the mouse CAMK4(P08414) (Met1-Tyr469) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Predicted N-Term: Gly
Pureté > 95 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Kinase activity untested
Nom de la protéine
Sujet Synonyms: A430110E23Rik,AI666733,CaMKIV,CaMKIV/Gr,D18Bwg0362e
UniProt P08414
Pathways EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Production of Molecular Mediator of Immune Response, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Date de péremption 12 months
Images (Fournisseur)
 image for Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-469) protein (ABIN3216431) Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-469) protein
Background publications Wissing, Jänsch, Nimtz, Dieterich, Hornberger, Kéri, Wehland, Daub: "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." dans: Molecular & cellular proteomics : MCP, Vol. 6, Issue 3, pp. 537-47, 2007 (PubMed).

Anderson, Noeldner, Reece, Wadzinski, Means: "Regulation and function of the calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex." dans: The Journal of biological chemistry, Vol. 279, Issue 30, pp. 31708-16, 2004 (PubMed).

Blaeser, Ho, Prywes, Chatila: "Ca(2+)-dependent gene expression mediated by MEF2 transcription factors." dans: The Journal of biological chemistry, Vol. 275, Issue 1, pp. 197-209, 2000 (PubMed).

Melander Gradin, Marklund, Larsson, Chatila, Gullberg: "Regulation of microtubule dynamics by Ca2+/calmodulin-dependent kinase IV/Gr-dependent phosphorylation of oncoprotein 18." dans: Molecular and cellular biology, Vol. 17, Issue 6, pp. 3459-67, 1997 (PubMed).

Chatila, Anderson, Ho, Means: "A unique phosphorylation-dependent mechanism for the activation of Ca2+/calmodulin-dependent protein kinase type IV/GR." dans: The Journal of biological chemistry, Vol. 271, Issue 35, pp. 21542-8, 1996 (PubMed).

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