Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) (AA 1-708) (Active) protein (His tag)

Détails pour le produit réf. ABIN3218785
Nom de la protéine
Attributs du protein
AA 1-708
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Origine
Rat (Rattus)
29
12
4
2
2
2
2
1
1
Source
Human Cells
29
8
6
5
3
2
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Purification/Conjugué
Cette MMP 9 protéine est marqué à la His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
Options
Attributs du produit The secreted recombinant rat MMP9 comprises 700 amino acids and predicts a molecular mass of 77.8 kDa. The apparent molecular mass of the rat MMP9 is approximately 100-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Structure: A DNA sequence encoding the rat MMP9 (EDL96479.1) (Met 1-Pro 708) was expressed, fused with a polyhistidine tag at the C-terminus.
Predicted N-Term: Ala 20
Pureté > 95 % as determined by SDS-PAGE
niveau d'endotoxine < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >1000 pmoles/min/μg .
(Activation description: The proenzyme needs to be activated by APMA for an activated form)
Nom de la protéine
Poids moléculaire 77.8 kDa, 100-110 kDa
Pathways Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 50 mM MES, 100 mM NaCl, 1 mM CaCl2, 10 % Glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Stock -20 °C,-80 °C
Stockage commentaire Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Date de péremption 12 months
Produit citée dans: Siddiqi, Chen, Advani, Thai, Batchu, Alghamdi, White, Sood, Gibson, Connelly, Marsden, Advani: "CXCR4 promotes renal tubular cell survival in male diabetic rats: implications for ligand inactivation in the human kidney." dans: Endocrinology, Vol. 156, Issue 3, pp. 1121-32, 2015 (PubMed).