Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (Propeptide) (Active) Protéine

Détails pour le produit réf. ABIN413737
Nom de la protéine
  • DSI
  • ERBA2L
  • GIT
  • P4Hbeta
  • PDI
  • PDIA1
  • PHDB
  • PO4DB
  • PO4HB
  • PROHB
  • ERp59
  • Pdia1
  • Thbp
  • PDIR
  • p55
  • p58
  • prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • prolyl 4-hydroxylase subunit beta L homeolog
  • protein disulfide-isomerase
  • P4HB
  • P4hb
  • p4hb.L
  • LOC8281065
Attributs du protein
Propeptide
8
5
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Origine
Humain
24
6
3
1
1
1
1
1
1
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
19
15
3
2
1
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
Options
Pureté ≥95 % by SDS-PAGE
≥95 % by HPLC
Stérilité Sterile filtered
niveau d'endotoxine <0.1 ng/μg
Biological Activity Comment Specification / Test Method: Recombinant PDI is biological active. Reductase activity is 1.0 x 10e3 ?650nm/ min-2, by measuring the turbidity increase at 650 nm due to insulin reduction. The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time. Isomerase activity is 0.5 μmol active RNase A min-1 μmol PDI-1, according to the re-activation of reduced and denatured RNase A.
Nom de la protéine
Sujet Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. Recombinant Human PDI produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 508 amino acids and having a molecular mass of 57.1 kDa. The human PDI contains N-terminal 6xHis-tag and is purified by proprietary chromatographic techniques.

Alternative Names: Protein Disulfide Isomerase, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55
Poids moléculaire 57.1 kDa
ID gène 5034
UniProt P07237
Pathways Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstituted PDI should be stored in working aliquots at -20 °C. For long-term storage, it is recommended to add carrier protein (0.1 % HAS or BSA).
Buffer Sterile filtered and lyophilized with no additives
Conseil sur la manipulation Centrifuge the vial prior to opening.
Stock -20 °C
Date de péremption 12 months
Produit citée dans: Kothari, Sen, Pendurthi, Rao: "Bovine protein disulfide isomerase-enhanced tissue factor coagulant function: is phospholipid contaminant in it the real culprit?" dans: Blood, Vol. 111, Issue 6, pp. 3295-6, 2008 (PubMed).

Pendurthi, Ghosh, Mandal, Rao: "Tissue factor activation: is disulfide bond switching a regulatory mechanism?" dans: Blood, Vol. 110, Issue 12, pp. 3900-8, 2007 (PubMed).

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