Tumor Necrosis Factor (TNF) (Active) Protéine

Détails pour le produit réf. ABIN4889933
Nom de la protéine
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LOC103694380
  • tnfa
Origine
Humain
112
55
31
9
7
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
164
28
20
6
3
3
2
2
1
1
1
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Application
ELISA, In vitro Assay (in vitro), In vivo Studies (in vivo), Western Blotting (WB)
Options
Séquence MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Pureté ≥ 95%
Stérilité 0.2 μm filtered
niveau d'endotoxine ≤ 1.00 LAL (EUs/ug)
Biological Activity Comment
  • Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D
  • Bioactivity Acceptance Criteria ED50 (ng/ml): 2 ng/mL
  • Bioactivity Acceptance Criteria (Units/mg): 5 x 10^5 units/mg
Nom de la protéine
Sujet Tumor necrosis factor alpha (TNFa) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNFa signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNFa functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

Alternate Names: TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF
Poids moléculaire Monomer: 17.5 MW Monomer/Total (kDa)
UniProt P01375
Pathways Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Indications d'application Optimal working dilution should be determined by the investigator.
Commentaires

Media Components: Luria Broth

Restrictions For Research Use only
Format Lyophilized
Reconstitution Sterile water at 0.1 mg/mL

Store at 4 °C and avoid freezing.
Buffer Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing : 10 mM sodium phosphate,  pH 7.5
Agent conservateur Without preservative
Conseil sur la manipulation Centrifuge vial before opening.
Stock 4 °C,-20 °C,-80 °C
Stockage commentaire 12 months from date of receipt when stored at -20°C to-80°C as supplied. 1 month when stored at 4°C after reconstituting as directed.
Date de péremption 12 months
Images (Fournisseur)
 image for Tumor Necrosis Factor (TNF) (Active) protein (ABIN4889933) Tumor Necrosis Factor (TNF) (Active) protein
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