CD226 Protein (CD226) (AA 19-247) (Fc Tag,AVI tag,Biotin)

N° du produit ABIN4949020

Détail du produit

Antigène: CD226

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 19-247

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD226 protéine est marqué à la Fc Tag,AVI tag,Biotin.

Application: Functional Studies (Func)

Marque: MABSol®,PrecisionAvi

Séquence: AA 19-247

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Attributs du produit: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 54.4 kDa. The protein migrates as 66-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Détail du antigène

Antigène: CD226

Autre désignation: DNAM-1 (CD226 Produits)

Synonymes: CD226 Protein, cd226 Protein, zgc:158817 Protein, BC051526 Protein, DNAM-1 Protein, DNAM1 Protein, Pta1 Protein, TLiSA1 Protein, PTA1 Protein, CD226 molecule Protein, CD226 antigen Protein, CD226 Protein, cd226 Protein, Cd226 Protein

Sujet: DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

Poids moléculaire: 54.4 kDa

NCBI Accession: NP_006557

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints