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VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)

Origine: Humain Hôte: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
N° du produit ABIN4949042
  • Antigène
    VEGF 165
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 27-191
    Origine
    • 17
    • 3
    • 2
    Humain
    Source
    • 11
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification/Conjugué
    His tag,AVI tag,Biotin
    Application
    Functional Studies (Func)
    Marque
    MABSol®,PrecisionAvi
    Séquence
    AA 27-191
    Specificité
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Attributs du produit
    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 22.4 kDa (monomer). As a result of glycosylation, the protein migrates as 30 kDa (monomer) under reducing (R) condition, and 48-60 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
    Pureté
    >95 % as determined by SDS-PAGE.
    niveau d'endotoxine
    Less than 1.0 EU per μg by the LAL method.
  • Commentaires

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Conseil sur la manipulation
    Please avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
  • Keys, Wetter, Hang, Rutschmann, Russo, Mally, Steffen, Zuppiger, Müller, Schneider, Faridmoayer, Lin, Aebi: "A biosynthetic route for polysialylating proteins in Escherichia coli." dans: Metabolic engineering, Vol. 44, pp. 293-301, (2018) (PubMed).

  • Antigène
    VEGF 165
    Autre désignation
    VEGF165
    Sujet
    VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.
    Poids moléculaire
    22.4 kDa (monomer)
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