Nerve Growth Factor Protein (NGF) (AA 19-239) (His tag)

N° du produit ABIN5665881

Détail du produit

Antigène: Nerve Growth Factor (NGF)

Activité biologique: Active

Attributs du protein: AA 19-239

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette Nerve Growth Factor protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 97 %

Biological Activity Comment: Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to nerve growth factor (NGF), the cells respond over a period of a week by ceasing cell division and extending neurites (Greene and Tischler, 1976). The cells were grown in Ham's F12K containing 5% fetal calf serum and 10% horse serum on polylysine or collagen coated plates. When cells reached log phase growth , fresh medium was added together with 10ng/mL of NGF, then cells were observed by inverted microscope everyday. Cell division ceasing and differentiation of PC12 cells after incubation with NGF (10ng/mL) for 6 days was shown in Figure1.Control group which received no NGF displayed no neurite outgrowth and cells multiply rapidly.

Information d'application

Indications d'application: Isoelectric Point: 10.1

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: Nerve Growth Factor (NGF)

Abstract: NGF Produits

Synonymes: Beta-NGF Protein, HSAN5 Protein, NGFB Protein, Ngfb Protein, ngfb Protein, ns:zft386 Protein, xx:zft386 Protein, zgc:109730 Protein, beta-NGF Protein, hsan5 Protein, beta-ngf Protein, NGF Protein, ngf Protein, nerve growth factor Protein, nerve growth factor b (beta polypeptide) Protein, nerve growth factor S homeolog Protein, NGF Protein, Ngf Protein, ngfb Protein, ngf Protein, ngf.S Protein

Sujet: Alternative Names: NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor

Poids moléculaire: 30/33kDa

UniProt: P22894

Pathways: Regulation of Cell Size