NEU1 Protein (AA 141-286) (His tag)
-
- Antigène Voir toutes NEU1 Protéines
- NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))
- Activité biologique
- Active
- Attributs du protein
- AA 141-286
-
Origine
- Humain
-
Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette NEU1 protéine est marqué à la His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Attributs du produit
- Tag location: N-terminal His Tag
- Pureté
- > 90 %
- Biological Activity Comment
- NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of NEU with CTSA.
- Top Product
- Discover our top product NEU1 Protéine
-
-
- Indications d'application
- Isoelectric Point: 5.5
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Agent conservateur
- Dithiothreitol (DTT), Other preservative, ProClin
- Précaution d'utilisation
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
- Antigène
- NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))
- Autre désignation
- Neuraminidase (NEU) (NEU1 Produits)
- Synonymes
- NANH Protein, NEU Protein, SIAL1 Protein, MGC81958 Protein, AA407268 Protein, AA407316 Protein, Aglp Protein, Apl Protein, Bat-7 Protein, Bat7 Protein, G9 Protein, Map-2 Protein, Neu Protein, Neu-1 Protein, neuraminidase 1 Protein, neuraminidase 1 (lysosomal sialidase) L homeolog Protein, NEU1 Protein, neu1.L Protein, neu1 Protein, Neu1 Protein
- Classe de substances
- Influenza Protein
- Sujet
- Alternative Names: NEU1, SIAL1, Sialidase 1, Lysosomal Sialidase, Acetylneuraminyl hydrolase, G9 sialidase, N-acetyl-alpha-neuraminidase 1
- Poids moléculaire
- 26kDa
- UniProt
- Q99519
- Pathways
- SARS-CoV-2 Protein Interactome
-