Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag)

Détails pour le produit réf. ABIN5665891, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • PTH1
  • Pthp
  • PTH-(1-84)
  • Pth1
  • Pthr1
  • PTH
  • parathyroid hormone
  • parathyroid hormone S homeolog
  • PTH
  • Pth
  • pth.S
Attributs du protein
AA 32-115
15
5
3
3
2
2
1
1
1
1
1
Origine
Rat (Rattus)
43
4
4
3
3
3
3
2
1
1
1
Source
Escherichia coli (E. coli)
37
8
3
2
2
Activité biologique
Active
Purification/Conjugué
Cette PTH protéine est marqué à la MBP tag,His tag.
Application
Activity Assay (AcA), Cell Culture (CC)
Options
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N° du produit (Fournisseur)
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Attributs du produit Tag location: Two N-terminal Tags, His-tag and MBP-tag
Pureté > 90 %
Biological Activity Comment PTH (Parathyroid hormone) is a hormone secreted by the parathyroid glands that is important in bone remodeling. As reported, osteoblast-like cell lines, such as ROS 17/2.8, UMR106, SaOS, U2OS, MG63, that exhibit PTHR1, respond with increased proliferation to PTH. Rat PTH shares similarities with human PTH in amino acid sequence with the identity of 71.3%. Thus, a proliferation assay of rat recombinant PTH was conducted using U2OS cells. Briefly, U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PTH. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of U2OS cells after incubation with PTH for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant PTH for 48h. The result was shown in Figure 2. It was obvious that PTH increased cell viability of U2OS cells.
Nom de la protéine
Sujet Alternative Names: iPTH, Intact Parathyroid Hormone, Parathormone, Parathyrin
Poids moléculaire 59kDa
UniProt P04089
Pathways cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process
Indications d'application Isoelectric Point: 5.8
Restrictions For Research Use only
Format Lyophilized
Buffer 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
Agent conservateur Dithiothreitol (DTT), Other preservative, ProClin
Précaution d'utilisation This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Images (Fournisseur)
 image for Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag) (ABIN5665891) PTH (Parathyroid hormone) is a hormone secreted by the parathyroid glands that is imp...
 image for Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag) (ABIN5665891) Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag) (Image 2)
 image for Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag) (ABIN5665891) Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag) (Image 3)
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