Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

S100A6 Protein (AA 1-90) (His tag)

S100A6 Origine: Humain Hôte: Escherichia coli (E. coli) > 90 % AcA, CC Active
N° du produit ABIN5665898
  • Antigène Voir toutes S100A6 Protéines
    S100A6 (S100 Calcium Binding Protein A6 (S100A6))
    Activité biologique
    Active
    Attributs du protein
    AA 1-90
    Origine
    • 12
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Humain
    Source
    • 14
    • 5
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette S100A6 protéine est marqué à la His tag.
    Application
    Activity Assay (AcA), Cell Culture (CC)
    Attributs du produit
    Tag location: N-terminal His Tag
    Pureté
    > 90 %
    Biological Activity Comment
    S100A6 is a calcium-binding protein, which functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by Interacting with distinct RAGE (Receptor for Advanced Glycation End Products). Thus a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, recombinant human S100A6 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100A6 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of S100A6 and RAGE was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of S100A6 with RAGE.
    Top Product
    Discover our top product S100A6 Protéine
  • Indications d'application
    Isoelectric Point: 5.7
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Agent conservateur
    Dithiothreitol (DTT), Other preservative, ProClin
    Précaution d'utilisation
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Antigène
    S100A6 (S100 Calcium Binding Protein A6 (S100A6))
    Abstract
    S100A6 Produits
    Synonymes
    S100A6 Protein, 2A9 Protein, 5B10 Protein, CABP Protein, CACY Protein, PRA Protein, CALCYCLIN Protein, Cacy Protein, S100 calcium binding protein A6 Protein, uncharacterized LOC618256 Protein, S100 calcium binding protein A6 (calcyclin) Protein, S100A6 Protein, S100a6 Protein, LOC618256 Protein
    Sujet
    Alternative Names: S100-A6, 2A9, 5B10, CABP, CACY, PRA, MLN 4, Calcyclin, Growth factor-inducible protein 2A9, Prolactin receptor-associated protein
    Poids moléculaire
    16kDa
    UniProt
    P06703
    Pathways
    S100 Proteins
Vous êtes ici:
Support technique