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CXCL12 Protein (AA 23-89) (His tag,GST tag)

CXCL12 Origine: Humain Hôte: Escherichia coli (E. coli) > 97 % AcA, CC Active
N° du produit ABIN5665906
  • Antigène Voir toutes CXCL12 Protéines
    CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
    Activité biologique
    Active
    Attributs du protein
    AA 23-89
    Origine
    • 27
    • 12
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Source
    • 42
    • 4
    • 3
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette CXCL12 protéine est marqué à la His tag,GST tag.
    Application
    Activity Assay (AcA), Cell Culture (CC)
    Attributs du produit
    Tag location: N-terminal His and GST Tag
    Pureté
    > 97 %
    Biological Activity Comment
    SDF1 (stromal cell-derived factor 1), also known as C-X-C motif chemokine 12, is a chemokine protein that has chemotaxis active on T-lymphocytes and monocytes. It is thought that SDF1 stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3; thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of SDF1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and SDF1 (10ng/mL, 30ng/mL and 60ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x100) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). Result shows SDF1 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (x100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (x400). Statistical results were shown in Figure 2. The optimum chemotaxis of SDF1 occurs at 10ng/mL.
  • Indications d'application
    Isoelectric Point: 8.5
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Agent conservateur
    Dithiothreitol (DTT), Other preservative, ProClin
    Précaution d'utilisation
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Antigène
    CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
    Autre désignation
    Stromal Cell Derived Factor 1 (SDF1) (CXCL12 Produits)
    Synonymes
    IRH Protein, PBSF Protein, SCYB12 Protein, SDF1 Protein, TLSF Protein, TPAR1 Protein, CINC-1 Protein, Gro1 Protein, Pbsf Protein, Scyb12 Protein, Sdf1 Protein, Tlsf Protein, Tpar1 Protein, Fsp Protein, KC Protein, Mgsa Protein, N51 Protein, Scyb1 Protein, gro Protein, sdf-1 Protein, sdf1 Protein, xSDF-1 Protein, SDF-1 Protein, cxcl12 Protein, sdf-1a Protein, sdf1a Protein, wu:fa55e10 Protein, wu:fc16h12 Protein, wu:fj84c02 Protein, C-X-C motif chemokine ligand 12 Protein, C-X-C motif chemokine ligand 1 Protein, chemokine (C-X-C motif) ligand 12 Protein, chemokine (C-X-C motif) ligand 1 Protein, C-X-C motif chemokine ligand 12 L homeolog Protein, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) Protein, CXCL12 Protein, cxcl12 Protein, Cxcl1 Protein, Cxcl12 Protein, cxcl12.L Protein, cxcl12a Protein
    Sujet
    Alternative Names: CXCL12, PBSF, P-BSF, SCYB12, SDF1-A, SDF1-B, SDF1A, SDF1B, TLSF-A, TLSF-B, TPAR1, Pre-B Cell Growth-Stimulating Factor, Chemokine C-X-C-Motif Ligand 12
    Poids moléculaire
    40kDa
    UniProt
    P48061
    Pathways
    Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
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