Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

IL-21 Protein (AA 30-162) (Fc Tag,AVI tag,Biotin)

IL21 Origine: Humain Hôte: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
N° du produit ABIN5674598
  • Antigène Voir toutes IL-21 (IL21) Protéines
    IL-21 (IL21) (Interleukin 21 (IL21))
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 30-162
    Origine
    • 32
    • 9
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 25
    • 15
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification/Conjugué
    Cette IL-21 protéine est marqué à la Fc Tag,AVI tag,Biotin.
    Marque
    PrecisionAvi
    Séquence
    AA 30-162
    Specificité
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Attributs du produit
    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 43.9 kDa. The protein migrates as 36 kDa and 48-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    Pureté
    >95 % as determined by SDS-PAGE.
    niveau d'endotoxine
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product IL21 Protéine
  • Commentaires

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5
    Conseil sur la manipulation
    Please avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
  • Antigène
    IL-21 (IL21) (Interleukin 21 (IL21))
    Autre désignation
    IL-21 (IL21 Produits)
    Synonymes
    IL-21 Protein, Za11 Protein, interleukin 21 Protein, IL21 Protein, Il21 Protein
    Sujet
    Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
    Poids moléculaire
    43.6 kDa
    NCBI Accession
    NP_001193935
    Pathways
    Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Vous êtes ici:
Support technique