PPM1F Protein (AA 1-454) (His tag)

N° du produit ABIN5853181

Détail du produit

Antigène: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Type de proteíne: Recombinant

Attributs du protein: AA 1-454

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PPM1F protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSMSSGAPQ KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV SGEADAASRA LTGSEDYLLL ACDGFFDVVP HQEVVGLVQS HLTRQQGSGL RVAEELVAAA RERGSHDNIT VMVVFLRDPQ ELLEGGNQGE GDPQAEGRRQ DLPSSLPEPE TQAPPRS

Pureté: > 90 % by SDS - PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1  mM DTT.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Autre désignation: PPM1F (PPM1F Produits)

Synonymes: 1110021B16Rik Protein, 4933427B07Rik Protein, CaMKPase Protein, Popx2 Protein, mKIAA0015 Protein, CAMKP Protein, FEM-2 Protein, POPX2 Protein, hFEM-2 Protein, protein phosphatase, Mg2+/Mn2+ dependent 1F Protein, protein phosphatase 1F (PP2C domain containing) Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1F Protein, PPM1F Protein, Ppm1f Protein

Sujet: PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. Recombinant human PPM1F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 52kDa (477aa)

NCBI Accession: NP_055449

UniProt: P49593

Pathways: Positive Regulation of Endopeptidase Activity