REV1 Protein (AA 51-256) (His tag)
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- Antigène Voir toutes REV1 Protéines
- REV1 (REV1, Polymerase (DNA Directed) (REV1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 51-256
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette REV1 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA
- Pureté
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product REV1 Protéine
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REV1, Polymerase (DNA Directed) (REV1) (AA 1097-1194) protein (GST tag)
REV1 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
REV1, Polymerase (DNA Directed) (REV1) (AA 1-1249) protein (Strep Tag)Crystallography grade REV1 Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
REV1, Polymerase (DNA Directed) (REV1) (AA 1-1251) protein (Strep Tag)Crystallography grade REV1 Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- REV1 (REV1, Polymerase (DNA Directed) (REV1))
- Autre désignation
- REV1 (REV1 Produits)
- Synonymes
- ARABIDOPSIS THALIANA HOMOLOG OF REVERSIONLESS 1 Protein, ATREV1 Protein, K23L20.9 Protein, K23L20_9 Protein, 1110027I23Rik Protein, AU022044 Protein, Rev1l Protein, REV1L Protein, DNA-directed DNA polymerase Protein, HIG1 hypoxia inducible domain family member 1C Protein, REV1, DNA directed polymerase Protein, REV1 Protein, HIGD1C Protein, Rev1 Protein
- Sujet
- REV1 contains a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Recombinant human REV1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Poids moléculaire
- 25.2 kDa (227aa)
- NCBI Accession
- NP_057400
- UniProt
- Q9UBZ9
- Pathways
- Réparation de l'ADN, Regulation of Actin Filament Polymerization, Production of Molecular Mediator of Immune Response
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