DNMT3L Protein (AA 1-386) (His tag)

N° du produit ABIN5853724

Détail du produit

Antigène: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-386

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette DNMT3L protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSEFMAAIP ALDPEAEPSM DVILVGSSEL SSSVSPGTGR DLIAYEVKAN QRNIEDICIC CGSLQVHTQH PLFEGGICAP CKDKFLDALF LYDDDGYQSY CSICCSGETL LICGNPDCTR CYCFECVDSL VGPGTSGKVH AMSNWVCYLC LPSSRSGLLQ RRRKWRSQLK AFYDRESENP LEMFETVPVW RRQPVRVLSL FEDIKKELTS LGFLESGSDP GQLKHVVDVT DTVRKDVEEW GPFDLVYGAT PPLGHTCDRP PSWYLFQFHR LLQYARPKPG SPRPFFWMFV DNLVLNKEDL DVASRFLEME PVTIPDVHGG SLQNAVRVWS NIPAIRSRHW ALVSEEELSL LAQNKQSSKL AAKWPTKLVK NCFLPLREYF KYFSTELTSS L

Pureté: > 90 % by SDS - PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Autre désignation: DNMT3L (TRDMT1 Produits)

Synonymes: DNMT3L Protein, D6Ertd14e Protein, ecat7 Protein, DNA methyltransferase 3 like Protein, DNA (cytosine-5-)-methyltransferase 3-like Protein, DNMT3L Protein, Dnmt3l Protein

Sujet: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. DNMT3L is a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. It also mediates transcriptional repression through interaction with histone deacetylase 1. Recombinant human DNMT3L protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Poids moléculaire: 46.2kDa (411aa) confirmed by MALDI-TOF

NCBI Accession: NP_787063

UniProt: Q9UJW3