CST5 Protein (AA 26-142) (His tag)

N° du produit ABIN5854281

Détail du produit

Antigène: CST5 (Cystatin D (CST5))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 26-142

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette CST5 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: QSRTLAGGIH ATDLNDKSVQ RALDFAISEY NKVINKDEYY SRPLQVMAAY QQIVGGVNYY FNVKFGRTTC TKSQPNLDNC PFNDQPKLKE EEFCSFQINE VPWEDKISIL NYKCRKVHHH HHH

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: The IC50 value is < 2.0nM. The inhibitory function of Cystatin 5 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In phosphate buffered saline (  pH 7.4) containing 10 % glycerol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: CST5 (Cystatin D (CST5))

Autre désignation: CST5 (CST5 Produits)

Synonymes: Cst10 Protein, Cystatin-5 Protein, cystatin D Protein, CST5 Protein, Cst5 Protein

Sujet: CST5, also known as Cystatin-D, is a novel member of the cystatin superfamily. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Two alleles of the cystatin D gene (CST5), encoding protein variants with either Cys or Arg as residue 26 in their 122-residue polypeptide chains, are present in the population. Recombinant human CST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 14.36kDa (123aa) (13.5-18kDa in SDS-PAGE under reducing conditions.)

NCBI Accession: NP_001891

UniProt: P28325