Adenylate Kinase 1 Protein (AK1) (AA 1-210) (His tag)

N° du produit ABIN7529381

Détail du produit

Antigène: Adenylate Kinase 1 (AK1)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-210

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette Adenylate Kinase 1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSMGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK

Pureté: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 150 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-Hcl buffer (  pH 8.0) containing 10 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Adenylate Kinase 1 (AK1)

Autre désignation: Ak1 (AK1 Produits)

Synonymes: ADK-1 Protein, AK1 Protein, CG17146 Protein, DAK1 Protein, Dak1 Protein, Dmel\\CG17146 Protein, adk1 Protein, bs34e10.y1 Protein, ak5 Protein, ADENYLATE KINASE 1 Protein, MLE2.3 Protein, MLE2_3 Protein, adenylate kinase 1 Protein, Ak-1 Protein, B430205N08Rik Protein, Ak 1 Protein, zgc:91930 Protein, Myokinase Protein, Adenylate kinase 1 Protein, adenylate kinase 1 Protein, Adk1 Protein, ak1 Protein, AK1 Protein, ADK1 Protein, Ak1 Protein

Sujet: Ak1, also known as Adenylate kinase isoenzyme 1 isoform 1, is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes.It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in Ak1 are the cause of a form of hemolytic anemia. Recombinant mouse Ak1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 25.5 kDa (233aa) confirmed by MALDI-TOF

NCBI Accession: NP_067490

UniProt: Q9R0Y5

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process