Adenylate Kinase 1 Protein (AK1) (AA 1-210) (His tag)
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- Antigène Voir toutes Adenylate Kinase 1 (AK1) Protéines
- Adenylate Kinase 1 (AK1)
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 1-210
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Origine
- Souris
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette Adenylate Kinase 1 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MGSMGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK
- Pureté
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 150 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product AK1 Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-Hcl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- Adenylate Kinase 1 (AK1)
- Autre désignation
- Ak1 (AK1 Produits)
- Synonymes
- ADK-1 Protein, AK1 Protein, CG17146 Protein, DAK1 Protein, Dak1 Protein, Dmel\\CG17146 Protein, adk1 Protein, bs34e10.y1 Protein, ak5 Protein, ADENYLATE KINASE 1 Protein, MLE2.3 Protein, MLE2_3 Protein, adenylate kinase 1 Protein, Ak-1 Protein, B430205N08Rik Protein, Ak 1 Protein, zgc:91930 Protein, Myokinase Protein, Adenylate kinase 1 Protein, adenylate kinase 1 Protein, Adk1 Protein, ak1 Protein, AK1 Protein, ADK1 Protein, Ak1 Protein
- Sujet
- Ak1, also known as Adenylate kinase isoenzyme 1 isoform 1, is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes.It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in Ak1 are the cause of a form of hemolytic anemia. Recombinant mouse Ak1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Poids moléculaire
- 25.5 kDa (233aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_067490
- UniProt
- Q9R0Y5
- Pathways
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
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