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IL16 Protein (AA 1203-1332) (His tag)

IL16 Origine: Humain Hôte: Escherichia coli (E. coli) > 97 % CC, AcA Active
N° du produit ABIN6239762
  • Antigène Voir toutes IL16 Protéines
    IL16 (Interleukin 16 (IL16))
    Activité biologique
    Active
    Attributs du protein
    AA 1203-1332
    Origine
    • 14
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain
    Source
    • 24
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette IL16 protéine est marqué à la His tag.
    Application
    Cell Culture (CC), Activity Assay (AcA)
    Attributs du produit
    Tag location: N-terminal His Tag
    Pureté
    > 97 %
    Biological Activity Comment
    Pro-IL16 (Interleukin16) is a 631 amino acid precursor molecule, which is then cleaved into different isoforms. Researches have shown that the recombinant human IL16, containing C-terminal 130 amino acids, has same bioactivity as the natural secreted human IL16. Besides, IL16 has been considered to stimulate the proliferation of Jurkat cells at low dose (10-9 M). Thus, a proliferation assay of recombinant human IL16 was conducted using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL16. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL16 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL16 significantly promoted cell proliferation of Jurkat cells.
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    Discover our top product IL16 Protéine
  • Indications d'application
    Isoelectric Point: 6
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Agent conservateur
    Dithiothreitol (DTT), Other preservative, ProClin
    Précaution d'utilisation
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Antigène
    IL16 (Interleukin 16 (IL16))
    Abstract
    IL16 Produits
    Synonymes
    IL16 Protein, LCF Protein, NIL16 Protein, PRIL16 Protein, prIL-16 Protein, mKIAA4048 Protein, IL-16 Protein, interleukin 16 Protein, IL16 Protein, Il16 Protein
    Sujet
    Alternative Names: LCF, PrIL-16, Lymphocyte Chemoattractant Factor, Pro-Interleukin 16
    Poids moléculaire
    17kDa
    UniProt
    Q14005
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