VEGFA Protein (AA 27-190) (His tag)

N° du produit ABIN6239764

Détail du produit

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Activité biologique: Active

Attributs du protein: AA 27-190

Origine: Rat

Source: HEK-293 Cells

Purification/Conjugué: Cette VEGFA protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 97 %

Biological Activity Comment: Vascular endothelial growth factor A (VEGF-A), a glycosylated mitogen, is known to be a vascular permeability factor and an endothelial cell growth factor secreted by the smooth muscle and endothelial cells. It has been reported that VEGF-A induces vascular permeability and growth, promotes monocyte/macrophage migration, and inhibits cell apoptosis and so on. To test the effect of VEGF-A on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFA. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC . Cell proliferation of ECV304 cells after incubation with VEGFA for 72h observed by inverted microscope was shown in Figure 1.

Information d'application

Indications d'application: Isoelectric Point: 7.9

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Abstract: VEGFA Produits

Synonymes: MVCD1 Protein, VEGF Protein, VPF Protein, Vegf Protein, Vegf120 Protein, Vegf164 Protein, Vegf188 Protein, Vpf Protein, vegf Protein, vegfa Protein, wu:fj82c06 Protein, VEGF-A Protein, VEGF164 Protein, eVEGF120 Protein, eVEGF164 Protein, vegf-a Protein, vpf Protein, vefg Protein, si:dkey-14d8.3 Protein, wu:fd42e02 Protein, vascular endothelial growth factor A Protein, vascular endothelial growth factor Aa Protein, vascular endothelial growth factor A L homeolog Protein, vascular endothelial growth factor Ab Protein, VEGFA Protein, Vegfa Protein, vegfaa Protein, vegfa.L Protein, vegfa Protein, vegfab Protein

Sujet: Alternative Names: VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A, Vascular Permeability Factor

Poids moléculaire: 22kDa

UniProt: P16612

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling