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AGE Protein (His tag)

AGE Origine: Toutes les espèces Hôte: Human > 90 % AcA, CC Active
N° du produit ABIN6239805
  • Antigène Voir toutes AGE Protéines
    AGE (Advanced Glycation End Products (AGE))
    Activité biologique
    Active
    Origine
    Toutes les espèces
    Source
    • 1
    Human
    Purification/Conjugué
    Cette AGE protéine est marqué à la His tag.
    Application
    Activity Assay (AcA), Cell Culture (CC)
    Attributs du produit
    Tag location: N-terminal His Tag
    Pureté
    > 90 %
    Biological Activity Comment
    Glucose and other reducing sugars can react non-enzymatically with the amino groups of proteins to form compounds called advanced glycation end products (AGEs). AGEs exert their cellular functions via the interaction with receptor for advanced glycation end products (RAGE). It has been reported that AGE stimulates the differentiation and proliferation of 3T3, thus a proliferation assay was conducted using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of AGE. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with AGE for 48h observed by inverted microscope was shown in Figure1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant AGE for 48h. The result was shown in Figure 2. It was obvious that AGE significantly increased cell viability of 3T3 cells.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 1 mM DTT, 5 % Trehalose and Proclin300.
    Agent conservateur
    Dithiothreitol (DTT), ProClin
    Précaution d'utilisation
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Antigène
    AGE (Advanced Glycation End Products (AGE))
    Autre désignation
    Advanced Glycation End Product (AGE) (AGE Produits)
    Synonymes
    RBP Protein, RNBP Protein, renin binding protein Protein, RENBP Protein
    Classe de substances
    Chemical
    Sujet
    Alternative Names: AGEs, Advanced Glycation End Products
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