IDO Protein (AA 2-403) (His tag)

N° du produit ABIN6239816

Détail du produit

Antigène: IDO (Indoleamine 2,3-Dioxygenase (IDO))

Activité biologique: Active

Attributs du protein: AA 2-403

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette IDO protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 97 %

Biological Activity Comment: IDO (Indoleamine 2,3-dioxygenase 1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. Thus, bioactivity of recombinant human IDO was measured through its ability to oxidize L-tryptophan to N-formyl-kynurenine, using Methylene Blue as indicator. Briefly, a mixture of 800μM L-tryptophan, 9000 units/mL catalase, and 40μM Methyene Blue with the addition of 80mM ascorbic acid reaction buffer was incubated with different concentrations of IDO, and the absorbance was read in 321nm every 1.5h. The result indicated that recombinant human IDO oxidize L-tryptophan Enzyme activity of recombinant human IDO.

Information d'application

Indications d'application: Isoelectric Point: 6.8

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % skl, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: IDO (Indoleamine 2,3-Dioxygenase (IDO))

Autre désignation: Indoleamine-2,3-Dioxygenase (IDO) (IDO Produits)

Synonymes: IDO Protein, IDO-1 Protein, INDO Protein, Ido Protein, Indo Protein, indoleamine 2,3-dioxygenase 1 Protein, IDO1 Protein, Ido1 Protein

Sujet: Alternative Names: CD107B, INDO, Indoleamine-Pyrrole 2,3 Dioxygenase

Poids moléculaire: 43kDa

UniProt: P14902