PDGFA Protein (AA 94-194) (His tag)

N° du produit ABIN6239819

Détail du produit

Antigène: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Activité biologique: Active

Attributs du protein: AA 94-194

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PDGFA protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 95 %

Biological Activity Comment: PDGFA (Platelet-derived growth factor subunit A) is a Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of PDGFA on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and PDGFA (1.5ng/mL, 7.5ng/mL and 15ng/mL diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37oC with 5%CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x40) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). Result shows PDGFA is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (x40) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (x400). Statistical results were shown in Figure 2. The optimum chemotaxis of PDGFA occurs at 1.5ng/mL.

Information d'application

Indications d'application: Isoelectric Point: 8.9

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Autre désignation: Platelet Derived Growth Factor Subunit A (PDGFA) (PDGFA Produits)

Synonymes: PDGF-A Protein, PDGF1 Protein, pdgfa Protein, pdgfa-2 Protein, zgc:100777 Protein, pdgf Protein, pdgf1 Protein, pdgf-a Protein, AA409063 Protein, CD142 Protein, Cf-3 Protein, Cf3 Protein, TF Protein, PDGFACP Protein, wu:fc32c12 Protein, zgc:153249 Protein, platelet derived growth factor subunit A Protein, platelet-derived growth factor alpha polypeptide a Protein, coagulation factor III Protein, platelet derived growth factor, alpha Protein, platelet derived growth factor subunit A S homeolog Protein, platelet-derived growth factor alpha polypeptide b Protein, PDGFA Protein, pdgfaa Protein, pdgfa Protein, F3 Protein, Pdgfa Protein, pdgfa.S Protein, pdgfab Protein

Sujet: Alternative Names: PDGF-A, PDGF1, Platelet Derived Growth Factor Alpha Polypeptide

Poids moléculaire: 17kDa

UniProt: P20033

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling