CLCA1 Protein (AA 416-670) (His tag)

N° du produit ABIN6239825

Détail du produit

Antigène: CLCA1 (Chloride Channel Accessory 1 (CLCA1))

Activité biologique: Active

Attributs du protein: AA 416-670

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CLCA1 protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 97 %

Biological Activity Comment: CLCA1 (Calcium-activated chloride channel regulator 1) is a member of the calcium sensitive chloride conductance protein family. This protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits. It has been reported that CLCA1 activates calcium-dependent chloride channel through the interaction with TMEM16A (anoctamin-1). Besides, there exits similarities between human and mouse TMEM16A in amino acid sequence with the identity of 89.66%. Thus, a functional ELISA assay was conducted to detect the association of recombinant human CLCA1 with recombinant mouse TMEM16A. Briefly, CLCA1 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TMEM16A-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CLCA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CLCA1 with TMEM16A was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of CLCA1 with TMEM16A

Information d'application

Indications d'application: Isoelectric Point: 7.1

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: CLCA1 (Chloride Channel Accessory 1 (CLCA1))

Abstract: CLCA1 Produits

Synonymes: CACC Protein, CACC1 Protein, CLCRG1 Protein, CaCC-1 Protein, GOB5 Protein, hCLCA1 Protein, hCaCC-1 Protein, Clca3 Protein, fi39d11 Protein, wu:fi39d11 Protein, AECC Protein, Clca1 Protein, Clca2 Protein, gob-5 Protein, gob5 Protein, chloride channel accessory 1 Protein, chloride channel Protein, chloride channel protein Protein, CLCA1 Protein, Clca1 Protein, clca1 Protein, clcA1 Protein

Sujet: Alternative Names: CaCC1, CLCRG1, Chloride Channel,Calcium Activated,Family Member 1, Calcium-activated chloride channel protein 1

Poids moléculaire: 28kDa

UniProt: A8K7I4