CYP1A2 Protein (AA 2-231) (His tag,GST tag)

N° du produit ABIN6239866

Détail du produit

Antigène: CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))

Activité biologique: Active

Attributs du protein: AA 2-231

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CYP1A2 protéine est marqué à la His tag,GST tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His and GST Tag

Pureté: > 95 %

Biological Activity Comment: CYP1A2 (Cytochrome P450 1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Besides, ASAH1 (Acid ceramidase) has been identified as an interactor of CYP1A2 through affinity capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ASAH1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1A2 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1A2 and ASAH1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CYP1A2 with ASAH1.

Information d'application

Indications d'application: Isoelectric Point: 8

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))

Autre désignation: Cytochrome P450 1A2 (CYP1A2) (CYP1A2 Produits)

Synonymes: CP12 Protein, P3-450 Protein, P450(PA) Protein, CYP1A2 Protein, CYPIA2 Protein, P450-PM4 Protein, Cyp1a1 Protein, P450-3 Protein, CYPD45 Protein, P-450d Protein, RATCYPD45 Protein, CYP1A5 Protein, CP450 Protein, CYP-IA2 Protein, cytochrome P450 family 1 subfamily A member 2 Protein, cytochrome P450 1A2 Protein, cytochrome P450 LM4 Protein, cytochrome P450, family 1, subfamily A, polypeptide 2 Protein, cytochrome P450, family 1, subfamily a, polypeptide 2 Protein, cytochrome P450 1A2-like Protein, CYP1A2 Protein, LOC100328937 Protein, Cyp1a2 Protein, VDBG_06455 Protein, LOC101825565 Protein, LOC102130662 Protein

Sujet: Alternative Names: CP12, P3-450, P450(PA), Cytochrome P450,Family 1,Subfamily A,Polypeptide 2

Poids moléculaire: 55kDa

UniProt: P05177