PON1 Protein (AA 35-206) (His tag)

N° du produit ABIN6239873

Détail du produit

Antigène: PON1 (Paraoxonase 1 (PON1))

Activité biologique: Active

Attributs du protein: AA 35-206

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PON1 protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 97 %

Biological Activity Comment: Paraoxonase 1 (PON1) is responsible for hydrolysing organophosphate pesticides and nerve gasses. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). Besides, Clusterin (CLU) has been identified as an interactor of PON1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON1 and recombinant human CLU. Briefly, PON1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CLU-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PON1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of PON1 and CLU was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of PON1 with CLU.

Information d'application

Indications d'application: Isoelectric Point: 5.1

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: PON1 (Paraoxonase 1 (PON1))

Abstract: PON1 Produits

Synonymes: esa Protein, pon Protein, pon2 Protein, MGC53915 Protein, MGC89222 Protein, PON1 Protein, ESA Protein, MVCD5 Protein, PON Protein, Pon Protein, paraoxonase 2 Protein, paraoxonase 1 Protein, pon2 Protein, PON1 Protein, Pon1 Protein

Sujet: Alternative Names: ESA, PON, Esterase A, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, A-esterase 1, Serum aryldialkylphosphatase 1

Poids moléculaire: 30kDa

UniProt: P27169