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CXCL2 Protein (AA 32-100) (His tag,GST tag)

CXCL2 Origine: Rat Hôte: Escherichia coli (E. coli) > 97 % AcA, CC Active
N° du produit ABIN6239880
  • Antigène Voir toutes CXCL2 Protéines
    CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))
    Activité biologique
    Active
    Attributs du protein
    AA 32-100
    Origine
    • 18
    • 12
    • 5
    • 2
    • 1
    • 1
    Rat
    Source
    • 25
    • 4
    • 2
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette CXCL2 protéine est marqué à la His tag,GST tag.
    Application
    Activity Assay (AcA), Cell Culture (CC)
    Attributs du produit
    Tag location: N-terminal His and GST Tag
    Pureté
    > 97 %
    Biological Activity Comment
    Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of GROb with DPP4.
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  • Indications d'application
    Isoelectric Point: 6.7
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Agent conservateur
    Dithiothreitol (DTT), Other preservative, ProClin
    Précaution d'utilisation
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Antigène
    CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))
    Autre désignation
    Growth Regulated Oncogene Beta (GROb) (CXCL2 Produits)
    Synonymes
    CINC-2a Protein, GRO2 Protein, GROb Protein, MGSA-b Protein, MIP-2a Protein, MIP2 Protein, MIP2A Protein, SCYB2 Protein, GRO3 Protein, Gro2 Protein, MIP-2 Protein, Mgsa-b Protein, Mip2 Protein, Scyb Protein, Scyb2 Protein, Mip-2 Protein, GROB Protein, MIP-2alpha Protein, C-X-C motif chemokine ligand 2 Protein, chemokine (C-X-C motif) ligand 2 Protein, CXCL2 Protein, Cxcl2 Protein
    Sujet
    Alternative Names: CXCL2, SCYB2, MIP2, GRO2, MIP2a, MGSAb, CINC2a, HSF, Macrophage inflammatory protein 2-alpha, Hematopoietic synergistic factor, Chemokine(C-X-C-Motif)ligand 2
    Poids moléculaire
    41kDa
    UniProt
    P30348
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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