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CAPNL1 Protein (AA 75-356) (His tag)

CAPNL1 Origine: Rat Hôte: Escherichia coli (E. coli) > 95 % AcA, CC Active
N° du produit ABIN6239882
  • Antigène Voir toutes CAPNL1 Protéines
    CAPNL1 (Calpain 1, Large Subunit (CAPNL1))
    Activité biologique
    Active
    Attributs du protein
    AA 75-356
    Origine
    • 4
    • 2
    • 2
    • 1
    Rat
    Source
    • 4
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette CAPNL1 protéine est marqué à la His tag.
    Application
    Activity Assay (AcA), Cell Culture (CC)
    Attributs du produit
    Tag location: N-terminal His Tag
    Pureté
    > 95 %
    Biological Activity Comment
    Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CAPN1 with STAT3.
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  • Indications d'application
    Isoelectric Point: 5.3
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Agent conservateur
    Dithiothreitol (DTT), Other preservative, ProClin
    Précaution d'utilisation
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Antigène
    CAPNL1 (Calpain 1, Large Subunit (CAPNL1))
    Autre désignation
    Calpain 1, Large Subunit (CAPN1) (CAPNL1 Produits)
    Synonymes
    CANP Protein, CANP1 Protein, CANPL1 Protein, muCANP Protein, muCL Protein, Capa-1 Protein, Capa1 Protein, mu-calpin Protein, si:ch211-203k12.4 Protein, si:dkeyp-101g1.3 Protein, calpain Protein, calpain 1 Protein, calpain 1, (mu/I) large subunit Protein, calpain 1, (mu/I) large subunit b Protein, calpain 11 Protein, CAPN1 Protein, Capn1 Protein, capn1 Protein, capn1b Protein, CAPN11 Protein
    Sujet
    Alternative Names: PIG30, CANPL1, MuCANP, MuCL, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium-activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain
    Poids moléculaire
    33kDa
    UniProt
    P97571
    Pathways
    Apoptose
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