CAPNL1 Protein (AA 387-694) (His tag)

N° du produit ABIN6239883

Cette protéine CAPNL1 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour CAPNL1 Protein (AA 387-694) (His tag) (ABIN6239883)

Antigène: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Activité biologique: Active

Origine: Rat

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Cell Culture (CC)

Pureté: > 90 %

Détail du produit

Attributs du protein: AA 387-694

Purification/Conjugué: Cette CAPNL1 protéine est marqué à la His tag.

Attributs du produit: Tag location: N-terminal His Tag

Biological Activity Comment: Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CAPN1 with STAT3.

Information d'application

Indications d'application: Isoelectric Point: 5.9

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Autre désignation: Calpain 1, Large Subunit (CAPN1)

Sujet: Alternative Names: PIG30, CANPL1, MuCANP, MuCL, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium-activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain

Poids moléculaire: 35kDa

UniProt: P97571

Pathways: Apoptose