CYP1B1 Protein (AA 374-516) (His tag)

N° du produit ABIN6239886

Détail du produit

Antigène: CYP1B1 (Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1))

Activité biologique: Active

Attributs du protein: AA 374-516

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CYP1B1 protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 97 %

Biological Activity Comment: Cytochrome P450 family 1 subfamily B member 1 (CYP1B1) is an enzyme, Which belongs to the cytochrome P450 superfamily. CYP1B1 can catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. Besides, Heat Shock Protein family B (small) member 2 (HSPb2) has been identified as an interactor of CYP1B1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1B1 and recombinant human HSPb2, CYP1B1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPb2-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1B1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1B1 and HSPb2 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CYP1B1 with HSPb2.

Information d'application

Indications d'application: Isoelectric Point: 7.9

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: CYP1B1 (Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1))

Autre désignation: Cytochrome P450 1B1 (CYP1B1) (CYP1B1 Produits)

Synonymes: CP1B Protein, CYPIB1 Protein, GLC3A Protein, P4501B1 Protein, P4501b1 Protein, CYP1B1 Protein, CYP1B Protein, zgc:136223 Protein, cytochrome P450 family 1 subfamily B member 1 Protein, cytochrome P450, family 1, subfamily b, polypeptide 1 Protein, cytochrome P450, family 1, subfamily B, polypeptide 1 Protein, cytochrome P450 1B1 Protein, CYP1B1 Protein, Cyp1b1 Protein, cyp1b1 Protein, LOC100719054 Protein

Sujet: Alternative Names: CP1B, GLC3A, Cytochrome P450,Family 1,Subfamily B,Polypeptide 1, Glaucoma 3,Primary Infantile

Poids moléculaire: 18/16kDa

UniProt: Q16678

Pathways: Steroid Hormone Biosynthesis