MFAP2 Protein (AA 6-162) (His tag)

N° du produit ABIN6239901

Cette protéine MFAP2 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour MFAP2 Protein (AA 6-162) (His tag) (ABIN6239901)

Antigène: MFAP2 (Microfibrillar Associated Protein 2 (MFAP2))

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Cell Culture (CC)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 6-162

Purification/Conjugué: Cette MFAP2 protéine est marqué à la His tag.

Attributs du produit: Tag location: N-terminal His Tag

Biological Activity Comment: Microfibrillar-associated protein 2 (MFAP2) is an O-glycosylated protein which excreted to the extracellular space and the extracellular matrix. MFAP2 combine biglycan and elastin to form a ternary complex. MFAP2 plays a key role in the support and distensibility of the juxtacanalicular region of these collector channels. It also can inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of MFAP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MFAP2 and recombinant human FBN1. Briefly, MFAP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FBN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MFAP2 and FBN1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of MFAP2 with FBN1.

Information d'application

Indications d'application: Isoelectric Point: 5.5

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: MFAP2 (Microfibrillar Associated Protein 2 (MFAP2))

Sujet: Alternative Names: MAGP1, Microfibril-associated glycoprotein 1

Poids moléculaire: 38kDa

UniProt: P55001