SIRT3 Protein (AA 127-303) (His tag)

N° du produit ABIN6239975

Détail du produit

Antigène: SIRT3 (Sirtuin 3 (SIRT3))

Activité biologique: Active

Attributs du protein: AA 127-303

Origine: Rat

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette SIRT3 protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 95 %

Biological Activity Comment: Sirtuin 3 (SIRT3), the NAD-dependent deacetylaseis is member of the mammalian sirtuin family of proteins. In human, sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. It also can regulate epigenetic gene silencing and suppress recombination of rDNA in yeast. SIRT3 expression in white and brown adipose tissue. Besides, X-Ray Repair Cross Complementing 6 (XRCC6) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant Rat SIRT3 and recombinant Rat XRCC6. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to XRCC6-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SIRT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and XRCC6 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of SIRT3 with XRCC6.

Information d'application

Indications d'application: Isoelectric Point: 6.2

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: SIRT3 (Sirtuin 3 (SIRT3))

Abstract: SIRT3 Produits

Sujet: Alternative Names: SIR2L3, Silent Mating Type Information Regulation 2 Homolog 3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, SIR2-like protein 3, Regulatory protein SIR2 homolog 3

Poids moléculaire: 22kDa

UniProt: C6ZII9