IgG Fc (AA 99-330) (Active) protein (AVI tag,Biotin)

N° du produit ABIN5674596

Détail du produit

Antigène: IgG Fc

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 99-330

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: AVI tag,Biotin

Marque: PrecisionAvi

Séquence: AA 99-330

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Attributs du produit: This protein carries an Avi tag at the C-terminus. The protein has a calculated MW of 28.4 kDa. The protein migrates as 36-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Référence

Mukundan, Guan, Singleton, Yang, Li, Parekkadan: "Artificial T Cell Mimetics to Combat Melanoma Tumor Growth." dans: American journal of advanced drug delivery, Vol. 6, Issue 1, pp. 21-32, (2018) (PubMed).

Détail du antigène

Antigène: IgG Fc

Classe de substances: Antibody

Sujet: Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.

Poids moléculaire: 28.4 kDa