CD45 Protein (AA 1031-1251) (His tag)

N° du produit ABIN6387391

Détail du produit

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Type de proteíne: Recombinant

Attributs du protein: AA 1031-1251

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CD45 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVMIA AQGPLKETIG DFWQMIFQRK VKVIVMLTEL KHGDQEICAQ YWGEGKQTYG DIEVDLKDTD KSSTYTLRVF ELRHSKRKDS RTVYQYQYTN WSVEQLPAEP KELISMIQVV KQKLPQKNSS EGNKHHKSTP LLIHCRDGSQ QTGIFCALLN LLESAETEEV VDIFQVVKAL RKARPGMVST FEQYQFLYDV IASTYPAQNG QVKKNNHQED KIEFDNE

Pureté: > 95 % by SDS - PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Autre désignation: PTPRC (PTPRC Produits)

Synonymes: PTPRC Protein, si:dkeyp-47c5.1 Protein, B220 Protein, CD45 Protein, CD45R Protein, GP180 Protein, L-CA Protein, LCA Protein, LY5 Protein, T200 Protein, Cd45 Protein, Ly-5 Protein, Lyt-4 Protein, loc Protein, Lca Protein, RT7 Protein, cd45 Protein, protein tyrosine phosphatase, receptor type C Protein, protein tyrosine phosphatase, receptor type, C Protein, receptor-type tyrosine-protein phosphatase C Protein, PTPRC Protein, ptprc Protein, LOC100074271 Protein, Ptprc Protein

Sujet: PTPRC, also known as CD45, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Recombinant human PTPRC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 29.6 kDa (257aa) confirmed by MALDI-TOF

NCBI Accession: NP_002829

UniProt: P08575

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling